Sun Fan, Fan Yingying, Dai Yingying, Tang Muhai, Du Jiangtao, Xu Anqi, Liu Meichen, Chu Qianqian, Yu Dianzhen, Tian Ye, Sun Xiyan, Ren Weiheng, Cai Pengli, Yan Zheng, De Saeger Sarah, Wang Cheng, Wu Aibo
SINH-UGENT-SJTU Joint Laboratory of Mycotoxin Research, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
Institute of Quality Standards & Testing Technology for Agro-Products, Xinjiang Academy of Agricultural Sciences, Key Laboratory of Agro-products Quality and Safety of Xinjiang, Laboratory of Quality and Safety Risk Assessment for Agro-Products (Urumqi), Ministry of Agriculture and Rural Affairs, Urumqi, China.
Food Chem. 2025 Oct 1;488:144912. doi: 10.1016/j.foodchem.2025.144912. Epub 2025 May 24.
Tomato (Solanum lycopersicum) is susceptible to Alternaria fungi, accumulating Alternaria toxins including tenuazonic acid (TeA). Previous studies have primarily focused on overall contamination levels; however, this study employs MALDI-MSI and LC-MS/MS techniques to provide the first detailed analysis of the spatial distribution and average levels of TeA in individual tomatoes. Among 77 lesioned processing tomato samples, the exceedance rate of TeA was notably high. MALDI-MSI reveals TeA's spatial distribution, while LC-MS/MS offers quantitative data but is affected by sample processing. Specially, We found internal fungal colonization causing outward lesions with decreasing core-to-surface TeA. Artificial inoculation experiments elucidate the infection dynamics of Alternaria alternata and TeA accumulation over time. The tgCotA line exhibited significant resistance to A. alternata and a significant reduction in TeA content, while the tgChitinase line showed an increase in TeA content. This study provides novel strategies for control of TeA in tomatoes and their derived products.
番茄（Solanum lycopersicum）易受链格孢属真菌侵害，会积累包括细交链孢菌酮酸（TeA）在内的链格孢毒素。以往研究主要关注总体污染水平；然而，本研究采用基质辅助激光解吸电离质谱成像（MALDI-MSI）和液相色谱-串联质谱（LC-MS/MS）技术，首次对单个番茄中TeA的空间分布和平均含量进行了详细分析。在77个有病变的加工番茄样本中，TeA的超标率显著较高。MALDI-MSI揭示了TeA的空间分布，而LC-MS/MS提供定量数据，但受样品处理影响。特别地，我们发现内部真菌定殖导致向外的病变，且从核心到表面TeA含量逐渐降低。人工接种实验阐明了链格孢的感染动态以及TeA随时间的积累情况。转谷氨酰胺酶（tgCotA）品系对链格孢表现出显著抗性，TeA含量显著降低，而转几丁质酶（tgChitinase）品系的TeA含量有所增加。本研究为控制番茄及其衍生产品中的TeA提供了新策略。
