Department of Chemistry "Giacomo Ciamician", University of Bologna, Via Selmi 2, 40126 Bologna, Italy.
Department of Life Science (Agro-Food Science Area), University of Modena and Reggio Emilia, Via G. Amendola 2 (Padiglione Besta), 42122 Reggio Emilia, Italy.
Food Res Int. 2018 Apr;106:677-685. doi: 10.1016/j.foodres.2018.01.032. Epub 2018 Feb 8.
A liquid chromatography-tandem mass spectrometry method for the determination of four Alternaria toxins, i.e. alternariol, alternariol monomethyl ether, tentoxin, and tenuazonic acid in tomato-based and fruit-based products was developed using a QuEChERs approach for the extraction of the mycotoxins. To optimise the QuEChERs extraction, several parameters were tested: types of QuEChERs pouches, sample weights, quantities of added water, use of dispersive SPE as a purification step, types of solvent and conditions of shaking. The method showed good linearity (R > 0.997) and precision (RSD% < 10) for all analytes. Tenuazonic acid showed very good recovery (98.8%-108.9%) for tomato-based products, as well as for fruit-based products. The method was successfully applied to 57 samples collected from the Italian market. Tenuazonic acid was found in appreciable concentrations in some products. The highest value was found in a tomato sauce sample (814 μg/kg).
建立了一种基于 QuEChERS 的液质联用方法，用于测定番茄制品和水果制品中的四种交链孢霉毒素，即交链孢酚、交链孢酚单甲醚、棒曲霉素和脱氧雪腐镰刀菌烯醇。为了优化 QuEChERS 提取方法，测试了几种参数：QuEChERS 小袋的类型、样品重量、加入水的量、使用分散固相萃取作为净化步骤、溶剂的类型和摇晃条件。该方法对所有分析物均表现出良好的线性（R>0.997）和精密度（RSD%<10）。棒曲霉素对番茄制品和水果制品的回收率非常好（98.8%-108.9%）。该方法成功应用于从意大利市场采集的 57 个样本。在一些产品中发现了相当浓度的脱氧雪腐镰刀菌烯醇。在一个番茄酱样品中发现了最高值（814μg/kg）。
