具有来自废水和临床样本的mcr-10_ter基因座的肠杆菌属中mcr-10质粒的比较研究：对抗菌药物耐药性和适应性的影响
Comparative study of mcr-10 plasmids in Enterobacter spp. with the mcr-10_ter locus from wastewater and clinical samples: Implications for antimicrobial resistance and fitness.
作者信息
Yu Liansheng, Kayama Shizuo, Hayashi Wataru, Sugawara Yo, Kawakami Sayoko, Sugai Motoyuki
机构信息
Antimicrobial Resistance Research Center, National Institute of Infectious Diseases, Japan Institute for Health Security, Higashimurayama-shi, Tokyo, Japan.
Antimicrobial Resistance Research Center, National Institute of Infectious Diseases, Japan Institute for Health Security, Higashimurayama-shi, Tokyo, Japan.
出版信息
J Glob Antimicrob Resist. 2025 May 28;44:40-48. doi: 10.1016/j.jgar.2025.05.021.
OBJECTIVES
The emergence and dissemination of mobile colistin resistance (mcr) genes among various Enterobacter species found in humans, animals, and the environment have attracted significant global attention. We report the emergence of mcr-10-carrying plasmids in Enterobacter spp. isolated from wastewater in Japan. This study aimed to characterize the genetic environment of mcr-10 detected in Japan.
METHODS
Wastewater samples were collected bimonthly from four municipal wastewater treatment plants in Hiroshima, Japan between October 2020 and August 2021, and three mcr-10-harboring isolates were recovered. These isolates were characterized by antimicrobial susceptibility testing, whole-genome sequencing, and comparative analysis of mcr-10-carrying plasmids.
RESULTS
The three colistin-resistant strains, JARB-T-NT00029, JARB-T-NT00194, and JARB-T-NT00318 belonged to sequence types 364, 32, and 486, respectively. The mcr-10 genes were located on 118-155 kb plasmids of the IncFIB(K), IncFIA(HI1), and IncFIB(pECLA)/IncFII(pECLA) replicon types. These plasmids showed high similarity to mcr-10-carrying plasmids found in Enterobacter spp. isolated from human samples reported in other countries across Asia and Europe. Further detailed comparative analysis revealed that an mcr-10_ter locus-containing fragment, including genes involved in colistin resistance and the promotion of gut colonization, was conserved in 14 of 30 (46.7%) strains isolated from human samples in Japan.
CONCLUSIONS
We reported colistin-resistant Enterobacter spp. harboring plasmids carrying the mcr-10. This is the first detailed characterization of the genetic structures of the mcr-10-carrying plasmids isolated from the environment in Japan. Our investigation suggests the potential spread of a combination of genes for colistin resistance and the promotion of gut colonization.
目的
在人类、动物和环境中发现的各种肠杆菌属物种中，移动性黏菌素耐药（mcr）基因的出现和传播已引起全球广泛关注。我们报告了在日本从废水中分离出的携带mcr - 10质粒的肠杆菌属细菌。本研究旨在对在日本检测到的mcr - 10的基因环境进行表征。
方法
2020年10月至2021年8月期间，每两个月从日本广岛的四个城市污水处理厂采集废水样本，并分离出三株携带mcr - 10的菌株。通过抗菌药物敏感性试验、全基因组测序以及对携带mcr - 10质粒的比较分析对这些菌株进行表征。
结果
三株黏菌素耐药菌株JARB - T - NT00029、JARB - T - NT00194和JARB - T - NT00318分别属于序列类型364、32和486。mcr - 10基因位于IncFIB(K)、IncFIA(HI1)和IncFIB(pECLA)/IncFII(pECLA)复制子类型的118 - 155 kb质粒上。这些质粒与在亚洲和欧洲其他国家报告的从人类样本中分离出的携带mcr - 10的质粒高度相似。进一步详细的比较分析表明，在日本从人类样本中分离出的30株菌株中的14株（46.7%）中，一个包含参与黏菌素耐药和促进肠道定植的基因的含mcr - 10_ter位点的片段是保守的。
结论
我们报告了携带mcr - 10质粒的黏菌素耐药肠杆菌属细菌。这是首次对从日本环境中分离出的携带mcr - 10质粒的遗传结构进行详细表征。我们的数据表明了黏菌素耐药基因和促进肠道定植基因组合的潜在传播。
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