Nobthai Panida, Ruekit Sirigade, Peerapongpaisarn Dutsadee, Sukhchat Prawet, Swierczewski Brett E, Ruamsap Nattaya, Lertsethtakarn Paphavee
Department of Bacterial and Parasitic Diseases, Walter Reed Army Institute of Research-Armed Forces Research Institute of Medical Sciences (WRAIR-AFRIMS), Bangkok 10400, Thailand.
Queen Sirikit Naval Hospital, Chonburi 20180, Thailand.
Antibiotics (Basel). 2025 Jun 10;14(6):596. doi: 10.3390/antibiotics14060596.
The emergence of colistin resistance poses a significant threat to its efficacy as a last-line treatment against multidrug-resistant Gram-negative bacterial infections. In this study, 178 multi-drug resistant (MDR) isolates collected from clinical samples at Queen Sirikit Naval Hospital, Chonburi, Thailand, were evaluated for colistin resistance. Of these, six were identified as gene carriers, mediating colistin resistance. Specifically, was detected in three isolates, was detected in one isolate, and and were detected in two isolates, designated AMR-0220 and AMR-0361. Whole-genome sequencing and bioinformatics analysis revealed that AMR-0220 and AMR-0361 belonged to ST410 and ST617 lineages, respectively. Both isolates carried multiple plasmids, with located on an IncX4-type plasmid that is closely related to previously reported -carrying IncX4 plasmids. In contrast, was identified on distinct plasmid backbones: an IncFIB-type plasmid in AMR-0220 and an IncFII-type plasmid in AMR-0361. Overall, our findings demonstrate that the genes found in isolates in this region are located on different mobile genetic elements, indicating the potential for a widespread dissemination of colistin resistance among Gram-negative bacteria throughout Thailand's healthcare system.
黏菌素耐药性的出现对其作为治疗多重耐药革兰氏阴性菌感染的最后一线药物的疗效构成了重大威胁。在本研究中，对从泰国春武里诗丽吉王后海军医院的临床样本中收集的178株多重耐药（MDR）菌株进行了黏菌素耐药性评估。其中，有6株被鉴定为介导黏菌素耐药性的基因携带者。具体而言，在3株菌株中检测到了 ，在1株菌株中检测到了 ，在2株分别命名为AMR-0220和AMR-0361的菌株中检测到了 和 。全基因组测序及生物信息学分析表明，AMR-0220和AMR-0361分别属于ST410和ST617谱系。这两株菌株均携带多个质粒， 位于一个IncX4型质粒上，该质粒与先前报道的携带 的IncX4质粒密切相关。相比之下， 在不同的质粒骨架上被鉴定出来：在AMR-0220中为IncFIB型质粒，在AMR-0361中为IncFII型质粒。总体而言，我们的研究结果表明，该地区分离出的菌株中发现的 基因位于不同的可移动遗传元件上，这表明黏菌素耐药性在泰国整个医疗系统的革兰氏阴性菌中存在广泛传播的可能性。
