Zaitoun Baheeja, Amamcharla J K
Dairy Products Technology Center (DPTC), California Polytechnic State University, San Luis Obispo, CA 93407.
Midwest Dairy Foods Research Center, Department of Food Science and Nutrition, University of Minnesota, St. Paul, MN 55108.
J Dairy Sci. 2025 Aug;108(8):8247-8258. doi: 10.3168/jds.2025-26411. Epub 2025 May 28.
During the production of dairy-based drinks or their early storage months, there are no established chemical or physical indicators to reliably predict whether a specific beverage will remain stable throughout its intended shelf life or undergo premature destabilization. Consequently, the industry relies on observing physical changes, such as increased viscosity or particle size, to assess the shelf stability of milk protein concentrate-based low-acid beverages. This study aims to evaluate the effectiveness of the electrical resistance tomography in liner configuration in detecting destabilization in high-protein beverages (HPB). A formulation containing 8% protein (wt/wt) was prepared using 2 lots of milk protein concentrate and other ingredients to validate the method. The formulation was retort sterilized and stored at room temperature for up to 225 d. At regular intervals, the unshaken beverage bottle was carefully divided into 4 equal layers: layer 1 (top) through layer 4 (bottom). Physicochemical properties, including total protein (TP), soluble proteins, viscosity, and particle size, were analyzed in the separated layers. On d 0, the sample was homogeneous, with a mean (±SD) conductivity of 0.0998 ± 0.00 µS/cm across all layers. However, a significant increase in mean conductivity was observed across all layers from d 14 to d 88, followed by a sudden decrease on d 109. This was succeeded by another significant increase, lasting until d 225. By d 225, the TP content in layer 4 had risen from 7.86 ± 0.09 to 9.82 ± 0.69, which also contributed to the observed increase in overall conductivity. Moreover, the change in conductivity showed a positive correlation with the change in TP content across the layers. These findings suggest that electrical resistance tomography is a promising tool for detecting physicochemical changes in HPB and could effectively identify early-stage sedimentation in HPB.
在基于乳制品的饮料生产过程中或其储存的最初几个月里,没有既定的化学或物理指标能够可靠地预测某一特定饮料在其预期保质期内是否会保持稳定,还是会过早地出现不稳定情况。因此,该行业依赖于观察物理变化,如粘度增加或颗粒尺寸增大,来评估基于乳蛋白浓缩物的低酸饮料的货架稳定性。本研究旨在评估线性配置的电阻层析成像技术在检测高蛋白饮料(HPB)不稳定情况方面的有效性。使用两批乳蛋白浓缩物和其他成分制备了一种含8%蛋白质(重量/重量)的配方,以验证该方法。该配方经过高温瞬时灭菌后,在室温下储存长达225天。每隔一段时间,将未摇晃的饮料瓶小心地分成4个相等的层:第1层(顶部)至第4层(底部)。对分离出的各层分析了包括总蛋白(TP)、可溶性蛋白、粘度和颗粒尺寸在内的理化性质。在第0天,样品是均匀的,所有层的平均(±标准差)电导率为0.0998±0.00µS/cm。然而,从第14天到第88天,所有层的平均电导率都出现了显著增加,随后在第109天突然下降。接着又出现了另一次显著增加,一直持续到第225天。到第225天,第4层中的TP含量从7.86±0.09上升到了9.82±0.69,这也导致了观察到的整体电导率增加。此外,电导率的变化与各层TP含量的变化呈正相关。这些发现表明,电阻层析成像技术是检测HPB中理化变化的一种有前景的工具,并且能够有效地识别HPB中的早期沉降现象。