Gu Bon-Hee, Jung Ho Young, Rim Chae-Yun, Kim Tae-Yong, Lee Sang-Jin, Choi Doo Young, Park Han-Ki, Kim Myunghoo
Life and Industry Convergence Research Institute, Pusan National University, Miryang, Korea.
Division of Allergy and Clinical Immunology, Department of Internal Medicine, School of Medicine, Kyungpook National University, Kyungpook National University Chilgok Hospital, Daegu, Korea.
Microb Biotechnol. 2025 Jun;18(6):e70173. doi: 10.1111/1751-7915.70173.
The gut microbiome plays a crucial role in maintaining intestinal homeostasis and influencing immune-mediated diseases. Human faecal microbiota transplantation (FMT) is often employed in murine models to investigate the role of human microbes in disease regulation, but methods for effective colonisation require refinement. This study aimed to assess the colonisation efficiency of human microbiota in a murine model using FMT with human faeces, focusing particularly on the impact of gut microbiota depletion via polyethylene glycol (PEG) and comparing oral-gastric gavage with enema administration routes. Our findings revealed that PEG-induced depletion enhanced human microbiome colonisation in mice. Oral-gastric gavage prolonged colonisation, while enema administration facilitated quicker resolution of dysbiosis, both inducing selective human microbial colonisation in a time-dependent manner. Notably, genera such as Bacteroides, Blautia, Medicaternibacter and Bifidobacteria were successfully colonised, whereas Roseburia, Anaerostipes, Anaerobutyricum and Faecalibacterium failed to establish in the murine gut post-FMT. These findings highlight the challenges of replicating human gut microbiota in murine models and underscore the importance of selecting appropriate FMT methods based on desired outcomes. This study provides valuable insights into the colonisation dynamics of human microbiota in mice, contributing to the development of more effective FMT strategies for disease treatment.
肠道微生物群在维持肠道稳态和影响免疫介导疾病方面发挥着关键作用。人类粪便微生物群移植（FMT）常用于小鼠模型，以研究人类微生物在疾病调节中的作用，但有效定植的方法需要改进。本研究旨在评估使用人粪便进行FMT的小鼠模型中人类微生物群的定植效率，特别关注聚乙二醇（PEG）导致的肠道微生物群耗竭的影响，并比较经口胃灌胃与灌肠给药途径。我们的研究结果表明，PEG诱导的耗竭增强了小鼠体内人类微生物群的定植。经口胃灌胃延长了定植时间，而灌肠给药促进了失调的更快恢复，两者均以时间依赖性方式诱导选择性人类微生物定植。值得注意的是，拟杆菌属、布劳特氏菌属、医学杆菌属和双歧杆菌属等菌属成功定植，而罗斯氏菌属、厌氧短杆菌属、厌氧丁酸菌属和粪杆菌属在FMT后的小鼠肠道中未能定植。这些发现凸显了在小鼠模型中复制人类肠道微生物群的挑战，并强调了根据预期结果选择合适的FMT方法的重要性。本研究为人类微生物群在小鼠体内的定植动态提供了有价值的见解，有助于开发更有效的疾病治疗FMT策略。
