Davies Bethan K, Mebarek Saida, Davies Owen G, Orriss Isabel R
Department of Clinical and Experimental Endocrinology, KU Leuven, Leuven, Belgium.
Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, Université de Lyon, Lyon, France.
Methods Mol Biol. 2025;2885:143-157. doi: 10.1007/978-1-0716-4306-8_8.
This chapter describes the isolation and characterization of extracellular vesicles (EVs) isolated from primary rodent osteoblasts and extracellular matrix. The key advantages of this assay are that it allows the isolation of EVs from the conditioned media of primary osteoblasts at varying stages of differentiation and subsequent characterization prior to downstream applications.
本章描述了从原代啮齿动物成骨细胞和细胞外基质中分离细胞外囊泡（EVs）并进行表征的方法。该检测方法的关键优势在于，它能够从处于不同分化阶段的原代成骨细胞条件培养基中分离出EVs，并在下游应用之前对其进行后续表征。
