成骨细胞的分离与培养。

Isolation and Culture of Osteoblasts.

作者信息

Bourne Lucie E, Orriss Isabel R

机构信息

Centre for Lifelong Health, School of Applied Sciences, University of Brighton, Brighton, UK.

Department of Comparative Biomedical Sciences, Royal Veterinary College, London, UK.

出版信息

Methods Mol Biol. 2025;2885:3-22. doi: 10.1007/978-1-0716-4306-8_1.

DOI:10.1007/978-1-0716-4306-8_1

PMID:40448753

Abstract

This chapter describes the isolation, culture, and staining of primary osteoblasts. The key advantages of this assay are that it allows direct measurement of bone matrix deposition and mineralization, as well as yielding good quantities of osteoblasts at defined stages of differentiation for molecular and histological analysis. While the focus is on rodent and human osteoblasts, this chapter will also cover the culture of osteoblasts from less conventional animal species.

摘要

本章介绍了原代成骨细胞的分离、培养和染色。该检测方法的主要优点在于，它能够直接测量骨基质沉积和矿化情况，并且在特定分化阶段产生大量成骨细胞，用于分子和组织学分析。虽然重点是啮齿动物和人类成骨细胞，但本章也将涵盖来自不太常见动物物种的成骨细胞培养。