Gómez Arianna Ericka, Salmon Phil, Baumgarten Nell, Rai Jyoti, Huber Philippe, Simmonds Susana P, Yase Rohda A, Reynolds Kiana A, Tang W Joyce, Kwon Ronald Young
Department of Orthopaedics and Sports Medicine, University of Washington, Seattle, WA, USA.
Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA, USA.
Methods Mol Biol. 2025;2885:575-595. doi: 10.1007/978-1-0716-4306-8_28.
Zebrafish and other small laboratory fish have emerged as important model systems for bone biomedical research. Micro-computed tomography (microCT) is a powerful approach to non-destructively visualize and quantify bone morphology, microarchitecture, and mineral density. While consensus methods for microCT scanning analysis have been established in other vertebrates including small rodents, the small size of zebrafish, their different anatomy, and their amenability to rapid-throughput and high-content approaches call for unique considerations. In this chapter, we describe key considerations for performing microCT scans in the zebrafish skeleton. We also describe different approaches for sample mounting, scan acquisition, and analysis that can be used depending on experimental objectives and outcomes. Finally, we describe a step-by-step protocol for microCT scanning and analysis of adult zebrafish using FishCuT, resulting in hundreds of descriptors of vertebral morphology and mineral density.
斑马鱼和其他小型实验鱼类已成为骨生物医学研究的重要模型系统。微计算机断层扫描（microCT）是一种强大的方法，可用于非破坏性地可视化和量化骨形态、微观结构和矿物质密度。虽然在包括小型啮齿动物在内的其他脊椎动物中已经建立了微CT扫描分析的共识方法，但斑马鱼的小尺寸、它们不同的解剖结构以及它们对高通量和高内涵方法的适应性需要独特的考虑。在本章中，我们描述了在斑马鱼骨骼中进行微CT扫描的关键考虑因素。我们还描述了根据实验目标和结果可使用的不同样本安装、扫描采集和分析方法。最后，我们描述了使用FishCuT对成年斑马鱼进行微CT扫描和分析的分步方案，可生成数百个椎体形态和矿物质密度的描述符。
