微计算机断层扫描（MicroCT）用于扫描和分析小鼠骨骼。
MicroCT for Scanning and Analysis of Mouse Bones.
机构信息
Department of Orthopaedic Surgery and Musculoskeletal Research Center, Washington University, Saint Louis, MO, USA.
出版信息
Methods Mol Biol. 2021;2230:169-198. doi: 10.1007/978-1-0716-1028-2_11.
The purpose of this Chapter is to present a detailed description of methods for performing bone Micro-Computed Tomography (microCT) scanning and analysis. MicroCT is an x-ray imaging method capable of visualizing bone at the micro-structural scale, that is, 1-100 µm resolution. MicroCT is the gold-standard method for assessment of 3D bone morphology in studies of small animals. As applied to the small bones of mice or rats, microCT can efficiently and accurately assess bone structure (e.g., cortical bone area [Ct.Ar]) and micro-structure (e.g., trabecular bone volume fraction [Tb.BV/TV]). The particular application described herein is for post mortem mouse femur specimens. The material presented should be generally applicable to many commercially available laboratory microCT systems, although some details are specific to the system used in our lab (Scanco mCT 40; SCANCO Medical AG, Bruttisellen, Switzerland).
本章的目的是详细介绍进行骨微观计算机断层扫描（microCT）扫描和分析的方法。microCT 是一种 X 射线成像方法，能够在微观结构尺度上观察骨骼，即分辨率为 1-100μm。microCT 是评估小动物 3D 骨骼形态的金标准方法。应用于小鼠或大鼠的小骨，microCT 可以高效、准确地评估骨骼结构（例如皮质骨面积[Ct.Ar]）和微观结构（例如，小梁骨体积分数[Tb.BV/TV]）。本文所描述的特定应用是死后小鼠股骨标本。本文介绍的材料通常适用于许多商业上可用的实验室 microCT 系统，尽管有些细节是特定于我们实验室使用的系统（Scanco mCT 40；SCANCO Medical AG，Brüttisellen，瑞士）。
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