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通过双光子显微镜对单个癌细胞归巢至小鼠骨骼的体外分析。

Ex Vivo Analysis of Single Cancer Cell Homing to Murine Bone via Two-Photon Microscopy.

作者信息

Allocca Gloria, Wang Ning

机构信息

Department of Experimental Oncology, IEO, European Institute of Oncology IRCCS, Milan, Italy.

Leicester Cancer Research Centre, Department of Genetics, Genomics and Cancer Sciences, University of Leicester, Leicester, UK.

出版信息

Methods Mol Biol. 2025;2885:597-605. doi: 10.1007/978-1-0716-4306-8_29.

DOI:10.1007/978-1-0716-4306-8_29
PMID:40448781
Abstract

Two-photon microscopy has gained widespread recognition as a potent tool for providing both qualitative and quantitative information in the field of bone research. This chapter outlines a detailed protocol for using two-photon microscopy to track the colonization of cancer cells to bone, using frozen bone samples derived from xenograft mouse models.

摘要

双光子显微镜作为一种在骨研究领域提供定性和定量信息的强大工具已获得广泛认可。本章概述了一个详细的方案，用于使用双光子显微镜追踪癌细胞在骨中的定植情况，该方案使用源自异种移植小鼠模型的冷冻骨样本。

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1
Ex Vivo Analysis of Single Cancer Cell Homing to Murine Bone via Two-Photon Microscopy.通过双光子显微镜对单个癌细胞归巢至小鼠骨骼的体外分析。
Methods Mol Biol. 2025;2885:597-605. doi: 10.1007/978-1-0716-4306-8_29.
2
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本文引用的文献

1
The bone metastasis niche in breast cancer-potential overlap with the haematopoietic stem cell niche .乳腺癌中的骨转移微环境——与造血干细胞微环境的潜在重叠
J Bone Oncol. 2019 Jun 7;17:100244. doi: 10.1016/j.jbo.2019.100244. eCollection 2019 Aug.
2
Mouse models for studying prostate cancer bone metastasis.用于研究前列腺癌骨转移的小鼠模型。
Bonekey Rep. 2016 Feb 17;5:777. doi: 10.1038/bonekey.2016.4. eCollection 2016.
3
Osteoclasts control reactivation of dormant myeloma cells by remodelling the endosteal niche.破骨细胞通过重塑骨内膜微环境来控制休眠骨髓瘤细胞的重新激活。
Nat Commun. 2015 Dec 3;6:8983. doi: 10.1038/ncomms9983.
4
The frequency of osteolytic bone metastasis is determined by conditions of the soil, not the number of seeds; evidence from in vivo models of breast and prostate cancer.溶骨性骨转移的频率取决于“土壤”条件，而非“种子”数量；来自乳腺癌和前列腺癌体内模型的证据
J Exp Clin Cancer Res. 2015 Oct 20;34:124. doi: 10.1186/s13046-015-0240-8.
5
Mitotic quiescence, but not unique "stemness," marks the phenotype of bone metastasis-initiating cells in prostate cancer.有丝分裂静止而非独特的“干性”标志着前列腺癌骨转移起始细胞的表型。
FASEB J. 2015 Aug;29(8):3141-50. doi: 10.1096/fj.14-266379. Epub 2015 Apr 17.
6
Prostate cancer cells preferentially home to osteoblast-rich areas in the early stages of bone metastasis: evidence from in vivo models.前列腺癌细胞在骨转移的早期阶段优先归巢到成骨细胞丰富的区域：来自体内模型的证据。
J Bone Miner Res. 2014 Dec;29(12):2688-96. doi: 10.1002/jbmr.2300.
7
Knocking at the brain's door: intravital two-photon imaging of autoreactive T cell interactions with CNS structures.叩响大脑之门：与中枢神经系统结构相互作用的自身反应性 T 细胞的活体双光子成像。
Semin Immunopathol. 2010 Sep;32(3):275-87. doi: 10.1007/s00281-010-0216-x. Epub 2010 Jul 11.
8
Detection of functional haematopoietic stem cell niche using real-time imaging.利用实时成像技术检测功能性造血干细胞龛位。
Nature. 2009 Jan 1;457(7225):97-101. doi: 10.1038/nature07639. Epub 2008 Dec 3.