Shirnoush Narges, Emamifar Aryou, Davati Nafiseh
Department of Food Science and Technology, Faculty of Food Industry, Bu-Ali Sina University, Hamedan, Iran.
Sci Rep. 2025 May 31;15(1):19161. doi: 10.1038/s41598-025-03832-9.
The nanoliposomes loaded with ferrous sulfate were prepared by thin-layer hydration sonication and coated with nano-chitosan. Chitosan coating (0.2% w/v) and various lecithin: ferrous sulfate ratios (5:1, 10:1, and 20:1 w/w) were characterized based on the antimicrobial activity, particle size, polydispersity index (PDI), zeta potential, and encapsulation efficiency. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of ferrous sulfate against Staphylococcus aureus ATCC 25,923, Bacillus cereus ATCC 11,778, Escherichia coli ATCC 2592, and Pseudomonas aeruginosa ATCC 9027 were 1000 and 1500 mg/mL, respectively. Nanoliposomes containing ferrous sulfate with a higher ratio of lecithin: ferrous sulfate (20:1 w/w) showed more potent antibacterial activity with the MIC and MBC values of 500 and 750 mg/mL against all test microorganisms. The particle size, PDI and zeta potential values of the nanoliposomes loaded with ferrous sulfate increased significantly compared to the free nanoliposomes (84.65 nm, 0.25, - 33.55 mV). Increasing the ratio of lecithin to ferrous sulfate from 5:1 to 20:1 (w/w) decreased the particle size (350.51 nm), PDI (0.22), and zeta potential (- 18.1 mV) and increased the encapsulation efficiency (84.6%) of ferrous sulfate loaded nanoliposomes (p < 0.05). Chitosan coating increased the antimicrobial activity of nanoliposomes toward positive values.
通过薄层水化超声法制备负载硫酸亚铁的纳米脂质体，并用纳米壳聚糖进行包衣。基于抗菌活性、粒径、多分散指数（PDI）、zeta电位和包封率，对壳聚糖包衣（0.2% w/v）和不同的卵磷脂：硫酸亚铁比例（5:1、10:1和20:1 w/w）进行了表征。硫酸亚铁对金黄色葡萄球菌ATCC 25923、蜡样芽孢杆菌ATCC 11778、大肠杆菌ATCC 2592和铜绿假单胞菌ATCC 9027的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）分别为1000和1500 mg/mL。含有较高卵磷脂：硫酸亚铁比例（20:1 w/w）的负载硫酸亚铁的纳米脂质体对所有测试微生物均表现出更强的抗菌活性，MIC和MBC值分别为500和750 mg/mL。与游离纳米脂质体（84.65 nm、0.25、-33.55 mV）相比，负载硫酸亚铁的纳米脂质体的粒径、PDI和zeta电位值显著增加。将卵磷脂与硫酸亚铁的比例从5:1提高到20:1（w/w）可降低粒径（350.51 nm）、PDI（0.22）和zeta电位（-18.1 mV），并提高负载硫酸亚铁的纳米脂质体的包封率（84.6%）（p<0.05）。壳聚糖包衣使纳米脂质体的抗菌活性提高到正值。
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