Song C S, Yu J H, Bai D H, Hester P Y, Kim K H
J Immunol. 1985 Nov;135(5):3354-9.
Simple methods for the generation, purification, and assay of antibodies to the alpha-subunit of insulin receptor from eggs of immunized hens have been described. Chicken antibodies against the alpha-subunit inhibit insulin binding to the receptor and stimulate glucose oxidation as well as autophosphorylation of the beta-subunit. Thus the properties of chicken antibodies are very similar to those of antibodies found in human autoimmune diseases and different from rabbit antibodies obtained against the same antigen.
已经描述了从免疫母鸡的卵中生成、纯化和检测胰岛素受体α亚基抗体的简单方法。针对α亚基的鸡抗体可抑制胰岛素与受体的结合，并刺激葡萄糖氧化以及β亚基的自身磷酸化。因此，鸡抗体的特性与人类自身免疫性疾病中发现的抗体非常相似，与针对相同抗原获得的兔抗体不同。
