Lv Rui, Wu Liyu, Tian Fuli, Bai Hongmei, Huang Dongke, Lei Yanqiu, Liu Fenrong
School of Chemistry and Chemical Engineering, Inner Mongolia University, Hohhot, 010000, China.
Key Laboratory of Medicinal and Edible Plants Resources of Hainan Province, Hainan Vocational University of Science and Technology, Haikou, 571126, China.
J Fluoresc. 2025 Jun 1. doi: 10.1007/s10895-025-04370-3.
Carbon quantum dots are a new type of fluorescent material with promising application prospects in bioimaging, photocatalysis, and metal ions detection. Biomass is a plentiful and renewable natural resource containing a large amount of carbon, hydrogen and oxygen, and is an ideal precursor for the preparation of carbon quantum dots. Although biomass-based carbon dots possess numerous advantages, there are still several challenges. Specifically, the luminescence mechanism remains unidentified. Additionally, how to control the surface functionalization of biomass-based carbon dots is also a challenge. In this work, nitrogen-doped carbon quantum dots were synthesized sheep manure with glycine under optimized hydrothermal conditions to enhance nitrogen-containing functional groups. The results showed that the nitrogen-doped carbon quantum dots were synthesized when sheep manure mixed with glycine in a mass ratio of 4:1 and reacted at 240 ℃ for 12 h exhibited a quantum yield of 10.63%, a fluorescence lifetime of 7.82 ns, and exceptional stability-retaining fluorescence intensity above 630,855 a.u. after 30 days of storage. Furthermore, the nitrogen-doped carbon quantum dots exhibit high selectivity and sensitivity to Fe (detection limit:4.7 μM, linear range: 0-200 μM) along with excellent bioimaging performance in L929 cells, demonstrating their dual-functional applications in both environmental sensing and bioimaging.
碳量子点是一种新型荧光材料，在生物成像、光催化和金属离子检测等方面具有广阔的应用前景。生物质是一种丰富的可再生自然资源，含有大量的碳、氢和氧，是制备碳量子点的理想前驱体。尽管基于生物质的碳量子点具有众多优点，但仍存在一些挑战。具体而言，发光机制尚不清楚。此外，如何控制基于生物质的碳量子点的表面功能化也是一个挑战。在这项工作中，在优化的水热条件下，用甘氨酸从羊粪中合成了氮掺杂碳量子点，以增强含氮官能团。结果表明，当羊粪与甘氨酸以质量比4:1混合并在240℃反应12小时时合成的氮掺杂碳量子点，其量子产率为10.63%，荧光寿命为7.82纳秒，并且具有出色的稳定性——储存30天后荧光强度保持在630855任意单位以上。此外，氮掺杂碳量子点对铁表现出高选择性和高灵敏度（检测限：4.7μM，线性范围：0 - 200μM），同时在L929细胞中具有出色的生物成像性能，证明了它们在环境传感和生物成像方面的双重功能应用。
