Kamboj Neha, Kumar Rahul, Kumar Navin, Pal Manoj, Gautam Pankaj
Department of Microbiology, Graphic Era (Deemed to be University), 566/6, Clement Town, Dehradun 248002, UK, India.
Department of Biotechnology, Graphic Era (Deemed to be University), 566/6, Clement Town, Dehradun 248002, UK, India.
Food Chem X. 2025 Apr 30;27:102511. doi: 10.1016/j.fochx.2025.102511. eCollection 2025 Apr.
In this study, whole protein hydrolysate (Madira, PRJ-1) of barnyard millet's seed was produced through a fermentation process using and employed as an antibacterial hydrolysate. The antibacterial properties of the protein hydrolysate were investigated against (Gram-positive) and (Gram-negative) bacterial strains, respectively, using well diffusion method. Strikingly, a significant level of inhibition was observed, revealing zones of inhibition 11 ± 1.2 mm and 12 ± 1.5 mm against Gram-positive bacteria and Gram-negative respectively. Additionally, the fermented protein hydrolysate demonstrated significant antiprotease activity, with notable percentages of trypsin inhibition. The findings indicated statistically significant ( < 0.05) inhibition of microbial growth, with minimum inhibitory concentrations (MICs) 120 μg/200 μL for and against the at pH 7.0. These results highlight the potential of whole protein hydrolysate (Madira, PRJ-1) of barnyard millet's seed as a promising bioactive hydrolysate with antimicrobial applications.
在本研究中，稗子种子的全蛋白水解物（Madira，PRJ - 1）通过发酵过程制备，并用作抗菌水解物。分别使用打孔扩散法研究了该蛋白水解物对金黄色葡萄球菌（革兰氏阳性）和大肠杆菌（革兰氏阴性）菌株的抗菌性能。令人惊讶的是，观察到显著水平的抑制作用，对革兰氏阳性菌和革兰氏阴性菌的抑菌圈分别为11±1.2毫米和12±1.5毫米。此外，发酵后的蛋白水解物表现出显著的抗蛋白酶活性，对胰蛋白酶有显著的抑制百分比。研究结果表明，在pH 7.0时，对金黄色葡萄球菌和大肠杆菌的微生物生长具有统计学显著（P < 0.05）的抑制作用，最低抑菌浓度（MICs）分别为120μg/200μL。这些结果突出了稗子种子全蛋白水解物（Madira，PRJ - 1）作为一种有前景的具有抗菌应用的生物活性水解物的潜力。
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