Zhu Qiong, Liu Yi, Zhou Juan, Tan Miduo, Xue Yaxin, Tao Yin
Department of Gynaecology, Zhuzhou Central Hospital, Zhuzhou, 412000, People's Republic of China.
Department of Central Laboratory, Zhuzhou Central Hospital, Zhuzhou, 412000, People's Republic of China.
Int J Womens Health. 2025 May 28;17:1533-1545. doi: 10.2147/IJWH.S515836. eCollection 2025.
The present study aimed to evaluated the expression profiles of the Hydrogen sulfide (HS)-synthesizing enzymes cystathionine β-synthase (CBS) and cystathionine γ-lyase (CSE) expression and attempted to establish its relationship to ovarian cancer (OV) drug resistance and prognosis.
The expression levels of cystathionine β-synthase () and cystathionine γ-lyase () were analyzed in OV from GSE26193 and GSE14764 datasets, and OVCAR8 cisplatin resistant cell line from GSE45553 datasets. The correlation between prognosis with and expression levels were analyzed in OV from GSE26193 dataset. Immunohistochemical (IHC) analysis of and expression, meanwhile Kaplan-Meier analyzed of corresponding prognosis in OV patients. The relative drug sensitivity of and genes via gene set cancer analysis. Ultimately, the correlations among OV immune cells, , and . The and expression levels, and pan-cancer prognosis using TCGA database.
and were significantly overexpressed in the OV and OVCAR8 cisplatin resistant cell line (GSE26193: p < 0.001 and p < 0.0001; GSE14764: p < 0.001 and p < 0.0001 IHC: p<0.0001 and p<0.0001; GSE45553: p < 0.0001 and p < 0.0001, respectively). Interestingly, is closely associated with OS and PFS in GSE26193 dataset and OV patients (GSE26193: PFS, p = 0.0391; OS, p = 0.0328. OV patients: PFS, p= 0.017; OS, p=0.030). The and expression levels were correlated with immune cells, CD4 T cells, neutrophils, macrophages, and dendritic cells (DC) and their respective infiltration scores significantly differed. Both and expression levels influenced pan-cancer prognosis.
HS overexpression affected the OS and PFS and could also have an impact on the drug resistance of OV. Pan cancer prognosis may vary with HS expression.
本研究旨在评估硫化氢（HS）合成酶胱硫醚β合酶（CBS）和胱硫醚γ裂解酶（CSE）的表达谱，并试图确定其与卵巢癌（OV）耐药性及预后的关系。
分析GSE26193和GSE14764数据集中OV以及GSE45553数据集中OVCAR8顺铂耐药细胞系中胱硫醚β合酶（）和胱硫醚γ裂解酶（）的表达水平。在GSE26193数据集中分析OV中与表达水平和预后的相关性。对和表达进行免疫组织化学（IHC）分析，同时对OV患者的相应预后进行Kaplan-Meier分析。通过基因集癌症分析评估和基因的相对药物敏感性。最终，分析OV免疫细胞、和之间的相关性。利用TCGA数据库分析和表达水平以及泛癌预后。
在OV和OVCAR8顺铂耐药细胞系中，和均显著过表达（GSE26193：p < 0.001和p < 0.0001；GSE14764：p < 0.001和p < 0.0001；IHC：p<0.0001和p<0.0001；GSE45553：分别为p < 0.0001和p < 0.0001）。有趣的是，在GSE26193数据集和OV患者中，与总生存期（OS）和无进展生存期（PFS）密切相关（GSE26193：PFS，p = 0.0391；OS，p = 0.0328。OV患者：PFS，p= 0.017；OS，p=0.030）。和表达水平与免疫细胞、CD4 T细胞、中性粒细胞、巨噬细胞和树突状细胞（DC）相关，且它们各自的浸润评分有显著差异。和表达水平均影响泛癌预后。
HS过表达影响OS和PFS，也可能对OV的耐药性产生影响。泛癌预后可能随HS表达而变化。
