利用标记辅助将白叶枯病和豇豆花叶病毒抗性导入优良豇豆品种C-152。
Marker assisted introgression of bacterial leaf blight and cowpea mosaic virus resistance into an elite cowpea cultivar C-152.
作者信息
Lohithaswa Hirenallur Chandappa, Dinesh Hebse Bhojappa, Talekar Sidramappa Channappa, Viswanatha Kannalli Paramashivaiah, Mallikarjuna Mallana Goudra, Sowmya Muntagodu Shreekanth, Krishna Thaggihalli Veeranna, Singh Poonam, Ambika Dasannanamalige Siddeshi
机构信息
AICRP on Pigeonpea, ZARS, University of Agricultural Sciences, Bangalore, Karnataka 560 065 India.
Kirkhouse Trust Laboratory, Department of Genetics and Plant Breeding, University of Agricultural Sciences, Bangalore, Karnataka 560 065 India.
出版信息
Mol Breed. 2025 May 28;45(6):52. doi: 10.1007/s11032-025-01570-4. eCollection 2025 Jun.
UNLABELLED
Cowpea is an important grain legume crop and a source of vegetarian protein. C-152, a popular and widely adapted variety of cowpea, became susceptible to major cowpea diseases, viz., bacterial leaf blight (BLB) and cowpea mosaic virus (CpMV). Thus, in the current investigation, we have introgressed BLB and CpMV resistance from V-16 and V-57817, respectively, to the C-152 variety. A marker assisted simultaneous and stepwise backcross breeding scheme was used to recover an improved version of C-152 with resistance to BLB and CpMV diseases. Foreground (CISP markers VuMt401 and VuMt397 for BLB and SSR markers MA15 and MA80 for CpMV) and background selections were practiced using gene-specific and recurrent genome specific (72 markers) polymorphic markers. Two independent BCF lines from each cross possessing and gene with maximum genome recovery of the C-152 were inter-crossed to derive an inter-cross (IC) F population. Among the 10 promising ICF progenies, the line MC 17-2 (KBC-12), showing high yielding with resistance to BLB and CpMV, was selected. The superiority of the cowpea line MC 17-2 was evident in terms of a yield advantage of 8.68 to 28.68%, 9.30-47.00%, 1.10-8.10% over different check varieties in the initial varietal trial, advanced varietal trial (AVT)-I, and AVT-II, respectively. Further, the multi-location evaluation of KBC-12 (MC 17-2) with the check KBC-9 covering zones 5 and 6 of Karnataka reconfirmed the high-yielding potential and stability of KBC-12 across tested environments, as evident from AMMI and GGE biplots. Thus, the promising cowpea line KBC-12 was released for commercial cultivation in zones 5 and 6 in southern India during 2024 and can also be used as a donor (IC652010) of BLB and CpMV resistance. Our current study is one such examples that revealed the power of marker-assisted selection to deliver improved cultivars from lab to farmers' field.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s11032-025-01570-4.
未标记
豇豆是一种重要的食用豆类作物，也是素食蛋白质的来源。C-152是一种广受欢迎且适应性广泛的豇豆品种，但它对主要的豇豆病害，即细菌性叶斑病（BLB）和豇豆花叶病毒（CpMV）变得易感。因此，在当前的研究中，我们分别将来自V-16和V-57817的BLB和CpMV抗性导入到C-152品种中。采用了一种标记辅助的同时和逐步回交育种方案，以培育出对BLB和CpMV病害具有抗性的改良版C-152。利用基因特异性和轮回基因组特异性（72个标记）多态性标记进行前景选择（用于BLB的CISP标记VuMt401和VuMt397以及用于CpMV的SSR标记MA15和MA80）和背景选择。将每个杂交组合中具有C-152最大基因组恢复率且携带 和 基因的两个独立回交一代（BCF）株系进行杂交，以获得一个杂交（IC）F群体。在10个有前景的ICF后代中，选择了表现出高产且对BLB和CpMV具有抗性的MC 17-2（KBC-12）株系。在初始品种试验、高级品种试验（AVT）-I和AVT-II中，豇豆品系MC 17-2相对于不同对照品种的产量优势分别为8.68%至28.68%、9.30%至47.00%、1.10%至8.10%，其优势明显。此外，KBC-12（MC 17-2）与对照KBC-9在卡纳塔克邦第5和第6区的多点评估再次证实了KBC-12在不同测试环境中的高产潜力和稳定性，这从加性主效应和乘积交互作用（AMMI）分析和基因型与基因型互作（GGE）双标图中可以明显看出。因此，有前景的豇豆品系KBC-12于2024年在印度南部第5和第6区被批准用于商业种植，并且也可作为BLB和CpMV抗性的供体（IC652010）。我们目前的研究就是这样一个例子，揭示了标记辅助选择从实验室到农民田间培育改良品种的强大作用。
补充信息
在线版本包含可在10.1007/s11032-025-01570-4获取的补充材料。
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