Mannur D M, Babbar Anita, Thudi Mahendar, Sabbavarapu Murali Mohan, Roorkiwal Manish, Yeri Sharanabasappa B, Bansal Vijay Prakash, Jayalakshmi S K, Singh Yadav Shailendra, Rathore Abhishek, Chamarthi Siva K, Mallikarjuna Bingi P, Gaur Pooran M, Varshney Rajeev K
1Agricultural Research Station, University of Agricultural Sciences (UAS)-Raichur, Kalaburagi, Karnataka 585 101 India.
Jawaharlal Nehru Krishi Vishwa Vidyalaya (JNKVV), Jabalpur, Madhya Pradesh 482 004 India.
Mol Breed. 2019;39(1):2. doi: 10.1007/s11032-018-0908-9. Epub 2018 Dec 28.
Annigeri 1 and JG 74 are elite high yielding desi cultivars of chickpea with medium maturity duration and extensively cultivated in Karnataka and Madhya Pradesh, respectively. Both cultivars, in recent years, have become susceptible to race 4 of Fusarium wilt (FW). To improve Annigeri 1 and JG 74, we introgressed a genomic region conferring resistance against FW race 4 () through marker-assisted backcrossing using WR 315 as the donor parent. For foreground selection, TA59, TA96, TR19 and TA27 markers were used at Agricultural Research Station, Kalaburagi, while GA16 and TA96 markers were used at Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur. Background selection using simple sequence repreats (SSRs) for the cross Annigeri 1 × WR 315 in BCF and BCF lines resulted in 76-87% and 90-95% recurrent parent genome recovery, respectively. On the other hand, 90-97% genome was recovered in BCF lines in the case of cross JG 74 × WR 315. Multilocation evaluation of 10 BCF lines derived from Annigeri 1 provided one superior line referred to as Super Annigeri 1 with 8% increase in yield and enhanced disease resistance over Annigeri 1. JG 74315-14, the superior line in JG 74 background, had a yield advantage of 53.5% and 25.6% over the location trial means in Pantnagar and Durgapura locations, respectively, under Initial Varietal Trial of All India Coordinated Research Project on Chickpea. These lines with enhanced resistance and high yield performance are demonstration of successful deployment of molecular breeding to develop superior lines for FW resistance in chickpea.
安尼杰里1号和JG 74是鹰嘴豆的优良高产本地品种，生育期适中，分别在卡纳塔克邦和中央邦广泛种植。近年来，这两个品种都对镰刀菌枯萎病4号生理小种（FW）变得易感。为了改良安尼杰里1号和JG 74，我们以WR 315为供体亲本，通过标记辅助回交导入了一个赋予对FW 4号生理小种抗性的基因组区域（）。在前景选择中，在卡拉布尔吉农业研究站使用了TA59、TA96、TR19和TA27标记，而在贾巴尔普尔贾瓦哈拉尔尼赫鲁农业大学使用了GA16和TA96标记。对安尼杰里1号×WR 315杂交组合的BCF和BCF株系使用简单序列重复（SSR）进行背景选择，分别使轮回亲本基因组恢复率达到76 - 87%和90 - 95%。另一方面，在JG 74×WR 315杂交组合的BCF株系中，基因组恢复率为90 - 97%。对来自安尼杰里1号的10个BCF株系进行多点评估，得到一个优良株系，称为超级安尼杰里1号，其产量比安尼杰里1号提高了8%，抗病性增强。JG 74背景下的优良株系JG 74315 - 14，在鹰嘴豆全印度协调研究项目的初始品种试验中，在潘特纳加尔和杜尔加普拉试验点的产量分别比当地试验平均值高53.5%和25.6%。这些具有增强抗性和高产性能的株系证明了成功应用分子育种培育出鹰嘴豆抗FW优良株系。
