Antioxidant and protective effects of methanolic leaf extract against CCl4-induced hepatotoxicity and renotoxicity in albino rats.

BACKGROUND

There is growing interest in natural antioxidants, particularly (), because of their potential to protect against oxidative stress-induced organ damage and their intriguing historical use in treating various medical conditions.

AIM

This pioneering study examined the unique antioxidant, hepatoprotective, and renoprotective effects of methanolic leaf extract on carbon tetrachloride (CCl)-induced toxicity in albino rats.

METHODS

Thirty albino rats (100-120 g) were used. The 2.2 di-(4-tretoctylphenyl)-1-picrylhydrazyl radical scavenging assay was used to evaluate the antioxidant properties of extract. This study examined the protective effects of 150 and 300 mg/kg leaf extract against CCl-induced toxicity. Body weight, hematological markers, and kidney and liver function were assessed. Histological analysis of liver and kidney tissue was performed.

RESULTS

The radical scavenging activity of was a remarkable 85.02 ± 0.01 RSA%, compared to 66.83 ± 0.02 RSA% for gallic acid, with IC values of 58.3 and 1.22 μg/ml, respectively. treatment significantly decreased alanine transaminase and aspartate transaminase activities relative to CCl-induced toxicity, a finding of significance in pharmacology and toxicology.

CONCLUSION

The methanolic extract has an IC value of 58.3 μg/ml, demonstrating potent natural antioxidant properties that mitigate hepatotoxicity and renotoxicity induced by CCl.