Kabbashi Ahmed Saeed, Eltawaty Salwa Abdulla, Ismail Amar Mohamed, Elshikh Ahmed Abdelhhafiz, Alrasheid Ayat Ahmed, Elmahi Rawan Ahmed, Koko Waleed S, Osman Elbadri Elamin
Department of Biomedical Science Faculty of Pharmacy Omar Al-Mukhtar University, Al-Bayda, Libya.
Department of Botany Faculty of Science and Technology Omdurman Islamic University, Omdurman, Sudan.
J Toxicol. 2024 Aug 27;2024:5539386. doi: 10.1155/2024/5539386. eCollection 2024.
To investigate the antioxidant and hepatoprotective effects of ethanolic () seed extract on carbon tetrachloride (CCl)-induced hepatotoxicity in albino rats.
Forty-eight albino rats weighing (100-150 g) were used for hepatoprotective and toxicity experiments. Antioxidant activity was determined using the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay. The toxicity of seeds on the liver was evaluated by examining wellness parameters, body weight, and liver histological sections. The protective effects of 50 mg/kg and 100 mg/kg of seed extract on CCl-induced hepatotoxicity were investigated by evaluating hematological, renal, and liver function parameters, body weight, and liver histological sections.
The antioxidant activity of the ethanolic extract was (92 ± 0.03 RSA %) compared with (91 ± 0.01 RSA %) of propyl gallate, and the IC was (8.3 ± 0.01 g/ml) and (14.1 ± 0.01 g/ml). No changes were observed in the health indicators, body weights, and liver histological sections following oral administration of 50 mg/kg and 100 mg/kg of seed extracts. Treatment with seed extract significantly reduced alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), blood sugar, and urea levels compared with those in the CCl-treated group.
The IC of the ethanolic extract was 8.3 g/ml, and the extract is a potential source of natural antioxidants that protect against CCl-induced hepatotoxicity.
研究乙醇提取物对四氯化碳（CCl）诱导的白化大鼠肝毒性的抗氧化和保肝作用。
选用48只体重为（100 - 150克）的白化大鼠进行保肝和毒性实验。采用2,2 - 二苯基 - 1 - 苦基肼（DPPH）法测定抗氧化活性。通过检查健康参数、体重和肝脏组织切片来评估种子对肝脏的毒性。通过评估血液学、肾脏和肝脏功能参数、体重以及肝脏组织切片，研究50毫克/千克和100毫克/千克种子提取物对CCl诱导的肝毒性的保护作用。
乙醇提取物的抗氧化活性为（92±0.03 RSA%），与没食子酸丙酯的（91±0.01 RSA%）相比，其IC50为（8.3±0.01微克/毫升）和（14.1±0.01微克/毫升）。口服50毫克/千克和100毫克/千克种子提取物后，健康指标、体重和肝脏组织切片未观察到变化。与CCl处理组相比，种子提取物处理显著降低了丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、碱性磷酸酶（ALP）、血糖和尿素水平。
乙醇提取物的IC50为8.3微克/毫升，该提取物是一种潜在的天然抗氧化剂来源，可预防CCl诱导的肝毒性。
