Ethanolic Extract of Protects against CCl-Induced Hepatotoxicity via Antioxidant Capabilities in Albino Rats.

OBJECTIVE

To investigate the antioxidant and hepatoprotective effects of ethanolic () seed extract on carbon tetrachloride (CCl)-induced hepatotoxicity in albino rats.

METHODS

Forty-eight albino rats weighing (100-150 g) were used for hepatoprotective and toxicity experiments. Antioxidant activity was determined using the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay. The toxicity of seeds on the liver was evaluated by examining wellness parameters, body weight, and liver histological sections. The protective effects of 50 mg/kg and 100 mg/kg of seed extract on CCl-induced hepatotoxicity were investigated by evaluating hematological, renal, and liver function parameters, body weight, and liver histological sections.

RESULTS

The antioxidant activity of the ethanolic extract was (92 ± 0.03 RSA %) compared with (91 ± 0.01 RSA %) of propyl gallate, and the IC was (8.3 ± 0.01 g/ml) and (14.1 ± 0.01 g/ml). No changes were observed in the health indicators, body weights, and liver histological sections following oral administration of 50 mg/kg and 100 mg/kg of seed extracts. Treatment with seed extract significantly reduced alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), blood sugar, and urea levels compared with those in the CCl-treated group.

CONCLUSION

The IC of the ethanolic extract was 8.3 g/ml, and the extract is a potential source of natural antioxidants that protect against CCl-induced hepatotoxicity.