Firdaus Rizky Setia, Fadilah Nadiah Farah, Suwanti Lucia Tri, Arwati Heny, Puspitasari Heni
Postgraduate Student of Universitas Airlangga, Surabaya, Indonesia.
Division of Veterinary Parasitology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.
Open Vet J. 2025 Apr;15(4):1599-1606. doi: 10.5455/OVJ.2025.v15.i4.11. Epub 2025 Apr 30.
Sacha inchi () seed is a rich source of polyunsaturated fatty acids, namely omega-3 and omega-6, which provide various health benefits, especially in the prevention of inflammatory diseases. Malaria, caused by species, triggers inflammasome activation, leading to the release of proinflammatory cytokines.
This study aimed to reveal the immunomodulatory potential effect of Sacha inchi seed oil (SISO) on parasite growth and proinflammatory cytokine expression (Interleukin-6, Il-8, and Il-12) in -infected mice.
Twenty-five BALB/c mice were divided into five groups, including the control group (G1), infected and untreated group (G2), and three infected groups treated with SISO at doses of 250 mg/kg body weight (G3), 500 mg/kg body weight (G4), and 1,000 mg/kg body weight (G5). Parasitemia levels were determined by calculating the percentage of infected red blood cells, whereas cytokine levels were measured using ELISA.
SISO treatment significantly reduced parasite growth in the 500 mg/kg body weight (18.12%) and 1,000 mg/kg body weight (17.12%) groups compared with the untreated group (30.18%). Levels of Il-8 and Il-12 showed significant differences ( < 0.05), with the highest levels recorded in the untreated group (G2), followed by the 250 mg/kg body weight therapy group (G3). The mice in the groups treated with 500 mg/kg body weight (G4) and 1,000 mg/kg body weight (G5) showed a decrease, and the value was even close to the control group (G1). However, no significant changes in Il-6 levels were observed across the groups.
Sacha inchi seed oil has the potential to modulate the immune response of -infected mice through the inhibition of parasite growth and reduction of the proinflammatory cytokine levels Il-12 and Il-8, depending on the dose. A dose of 500 mg/kg body weight effectively reduced parasite growth and the levels of observed proinflammatory cytokines.
美藤果（）种子富含多不饱和脂肪酸，即ω-3和ω-6，具有多种健康益处，尤其在预防炎症性疾病方面。由 物种引起的疟疾会触发炎性小体激活，导致促炎细胞因子释放。
本研究旨在揭示美藤果籽油（SISO）对感染 小鼠的寄生虫生长和促炎细胞因子表达（白细胞介素-6、Il-8和Il-12）的免疫调节潜在作用。
将25只BALB/c小鼠分为五组，包括对照组（G1）、感染未治疗组（G2）以及三个分别用250毫克/千克体重（G3）、500毫克/千克体重（G4）和1000毫克/千克体重（G5）的SISO治疗的感染组。通过计算感染红细胞的百分比来确定寄生虫血症水平，而细胞因子水平则使用酶联免疫吸附测定法进行测量。
与未治疗组（30.18%）相比，SISO治疗使500毫克/千克体重组（18.12%）和1000毫克/千克体重组（17.12%）的寄生虫生长显著降低。Il-8和Il-12水平显示出显著差异（<0.05），未治疗组（G2）记录的水平最高，其次是250毫克/千克体重治疗组（G3）。500毫克/千克体重组（G4）和1000毫克/千克体重组（G5）的小鼠水平下降，数值甚至接近对照组（G1）。然而，各组间Il-6水平未观察到显著变化。
美藤果籽油有潜力通过抑制寄生虫生长和降低促炎细胞因子Il-12和Il-8水平来调节感染 小鼠的免疫反应，具体取决于剂量。500毫克/千克体重的剂量有效降低了寄生虫生长和观察到的促炎细胞因子水平。
