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在印度尼西亚东爪哇利用间接酶联免疫吸附测定法作为检测住白细胞虫病的一种新方法的发现。

discovery utilizing indirect-ELISA in East Java, Indonesia as a new approach in detecting leucocytozoonosis.

作者信息

Suprihati Endang, Suwanti Lucia Tri, Hastutiek Poedji, Lastuti Nunuk Dyah Retno, Ito Akira, Himeno Naomi

机构信息

Division of Veterinary Parasitology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.

Hokkaido Green Pharma Co., Ltd, Sapporo, Japan.

出版信息

Open Vet J. 2025 Apr;15(4):1557-1564. doi: 10.5455/OVJ.2025.v15.i4.6. Epub 2025 Apr 30.

Abstract

BACKGROUND

Leucocytozoonosis, caused by , is a significant parasitic disease in poultry and can cause substantial economic losses in the broiler chicken industry while the golden standard of examination is currently absent.

AIM

This study aimed to detect the presence of in broiler chickens in East Java Province using two different diagnostic methods.

METHODS

Blood samples were taken from the brachial vein of death broiler chickens from several farms recorded as endemic areas of leucocytozoonosis in East Java, Indonesia. Parasite detection was performed by parasitological examination (blood smear) and ELISA serological examination.

RESULTS

The results showed a prevalence of was 13.43% based on blood smear examination, whereas ELISA serological examination showed a higher prevalence (27.78%). This difference in results indicates that serological tests have a higher sensitivity in detecting infections than parasitological methods.

CONCLUSION

These findings emphasize the importance of using appropriate diagnostic methods to control leucocytozoonosis in broiler chicken farms. Furthermore, the results of this study can serve as a basis for developing more effective control strategies against this disease in East Java Province.

摘要

背景

由[病原体名称未给出]引起的住白细胞虫病是家禽中一种重要的寄生虫病,在肉鸡行业可造成重大经济损失,而目前尚无检测的金标准。

目的

本研究旨在使用两种不同的诊断方法检测印度尼西亚东爪哇省肉鸡中[病原体名称未给出]的存在情况。

方法

从印度尼西亚东爪哇几个被记录为住白细胞虫病流行地区的农场中死亡肉鸡的臂静脉采集血样。通过寄生虫学检查(血涂片)和ELISA血清学检查进行寄生虫检测。

结果

基于血涂片检查,[病原体名称未给出]的流行率为13.43%,而ELISA血清学检查显示流行率更高(27.78%)。结果的这种差异表明,血清学检测在检测[病原体名称未给出]感染方面比寄生虫学方法具有更高的灵敏度。

结论

这些发现强调了使用适当诊断方法控制肉鸡场住白细胞虫病的重要性。此外,本研究结果可为东爪哇省制定更有效的该病防控策略提供依据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/65c1/12124798/6d2845653049/OpenVetJ-15-1557-g001.jpg

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