de Oliveira Jerusa M, Costa Maria P C, Perini Hugo F, Trevisan Rafael O, de Almeida Larissa I M, de Matos Samanta L M, de Sousa Isabella de O F, Ruiz Letícia C, Silva Leonardo E de A, Rodrigues Virmondes, de Oliveira Carlo J F, da Silva Marcos V, Anhezini Lucas, Silva Anielle Christine A
Strategic Materials Laboratory, Physics Institute, Federal University of Alagoas, Campus A. C. Simões. Av. Lourival Melo Mota, S/N, Tabuleiro do Martins, Maceió 57072-970, Alagoas, Brazil.
Laboratory of In Vivo Toxicity Analysis, Institute of Biological Sciences and Health, Federal University of Alagoas, Campus A. C. Simões. Av. Lourival Melo Mota, S/N, Tabuleiro do Martins, Maceió 57072-970, Alagoas, Brazil.
ACS Omega. 2025 May 15;10(20):20117-20128. doi: 10.1021/acsomega.4c09594. eCollection 2025 May 27.
This study presents the innovative application of simonkolleite (SM), its nanocomposite with ZnO nanocrystals (NCs), and ZnO nano-/microcrystals to explore their bactericidal and biocompatibility properties for potential biomedical applications. A systematic evaluation of antibacterial activity, antibiofilm efficacy, reactive oxygen species (ROS) production, and Drosophila melanogaster as an in vivo model was performed. The synthesis of SM, SM/ZnO nanocomposites (NCPs), and ZnO nano- and microcrystals was performed at different annealing temperatures: SM at 100 °C, SM/Zn NCPs (63.6% and 69.5% of ZnO NCs) at 250 and 500 °C, respectively, and ZnO NCs and microcrystals (MCs) at 500 and 1000 °C, respectively. Notably, ZnO NCs and MCs have demonstrated pronounced cytotoxicity in the D. melanogaster model, correlating with their ability to rapidly generate reactive oxygen species (ROS), contributing to oxidative stress and cellular damage. The SM/ZnO NCPs (250 °C) exhibited superior biocompatibility, suggesting that the SM partial conversion to ZnO may mitigate toxicity while retaining antimicrobial efficacy. Furthermore, subinhibitory concentrations of SM effectively inhibited biofilm formation, a critical factor in bacterial resistance, highlighting their potential applications in medical device coatings and antimicrobial therapies. Collectively, this research underscores the promising role of these materials in addressing antibiotic resistance and enhancing biocompatibility in biomedical applications.
本研究展示了锌羟氯铅矿（SM）、其与氧化锌纳米晶体（NCs）的纳米复合材料以及氧化锌纳米/微晶的创新应用，以探索它们在潜在生物医学应用中的杀菌和生物相容性特性。对其抗菌活性、抗生物膜功效、活性氧（ROS）生成以及以黑腹果蝇作为体内模型进行了系统评估。在不同退火温度下合成了SM、SM/ZnO纳米复合材料（NCPs）以及氧化锌纳米和微晶：SM在100°C下合成，SM/Zn NCPs（氧化锌NCs含量分别为63.6%和69.5%）分别在250°C和500°C下合成，氧化锌NCs和微晶（MCs）分别在500°C和1000°C下合成。值得注意的是，氧化锌NCs和MCs在黑腹果蝇模型中表现出明显的细胞毒性，这与其快速产生活性氧（ROS）的能力相关，导致氧化应激和细胞损伤。SM/ZnO NCPs（250°C）表现出优异的生物相容性，这表明SM部分转化为氧化锌可能会减轻毒性，同时保留抗菌功效。此外，亚抑制浓度的SM能有效抑制生物膜形成，这是细菌耐药性的一个关键因素，凸显了它们在医疗器械涂层和抗菌治疗中的潜在应用。总的来说，这项研究强调了这些材料在解决抗生素耐药性以及增强生物医学应用中的生物相容性方面的重要作用。
