Wang Jie, Bojikian Karine D, Chen Aiyin, Wei Ping, Liu Liang, Jia Yali, Huang David
Casey Eye Institute, Oregon Health & Science University, Portland, OR, USA.
Department of Biomedical Engineering, Oregon Health & Science University, Portland, OR, USA.
Transl Vis Sci Technol. 2025 Jun 2;14(6):5. doi: 10.1167/tvst.14.6.5.
The purpose of this study was to characterize the central macular ganglion cell layer plexus (GCLP) boundaries using projection-resolved optical coherence tomographic angiography (PR-OCTA) in healthy eyes.
Participants were scanned using a commercial OCTA system (Avanti; Optovue/Visionix Inc., Fremont, CA, USA) in a 6 × 6-mm area centered on foveal fixation.PR-OCTA algorithm was used to remove flow projection artifacts. The anterior GCLP boundary was marked at the nerve fiber layer (NFL)/ganglion cell layer (GCL) junction, and the posterior boundary with the intermediate capillaries plexuses (ICPs) was determined by searching the ganglion cell and inner plexiform layer (GCIPL) slab for the watershed depth with minimum vessel density (VD). The foveal avascular zone (FAZ) within a 1-mm diameter circle was excluded from the analysis because the retinal plexuses merge near the FAZ. A polynomial fit was used to model the relationship between VD and depth.
Thirty-eight eyes of 38 healthy participants (79% female subjects) were enrolled. The mean age and standard deviation were 59.6 ± 10.7 years. The watershed between the GCLP and ICP was located at 75% depth within the GCIPL throughout the macula. Analysis of macular sectors on a polar grid showed that GCLP VD was correlated with NFL, GCIPL, and macular ganglion cell complex (GCC) thicknesses (R2 = 0.26, 0.15, and 0.73, respectively, P < 0.01 for all). The correlation was significantly stronger for GCC.
We defined the anatomic GCLP slab in the macula on PR-OCTA, which is the anterior 75% of the combined GCIPL. Its density correlates best with GCC, which also contains the NFL, suggesting that it also supplies at least the posterior aspect of the NFL.
Mapping macular GCLP holds promise for evaluating perfusion, particularly in conditions such as glaucoma and optic neuropathies.
本研究旨在利用投影分辨光学相干断层扫描血管造影（PR-OCTA）对健康眼睛的黄斑中心神经节细胞层丛（GCLP）边界进行特征描述。
使用商用OCTA系统（Avanti；美国加利福尼亚州弗里蒙特市Optovue/Visionix公司）对参与者以黄斑中心凹为中心的6×6毫米区域进行扫描。采用PR-OCTA算法去除血流投影伪影。GCLP的前边界标记在神经纤维层（NFL）/神经节细胞层（GCL）交界处，后边界与中间毛细血管丛（ICP）的交界处通过在神经节细胞和内丛状层（GCIPL）层板中寻找血管密度（VD）最小的分水岭深度来确定。直径1毫米圆内的黄斑无血管区（FAZ）被排除在分析之外，因为视网膜丛在FAZ附近融合。使用多项式拟合来模拟VD与深度之间的关系。
纳入了38名健康参与者的38只眼睛（79%为女性）。平均年龄和标准差分别为59.6±10.7岁。GCLP和ICP之间的分水岭位于整个黄斑区GCIPL的75%深度处。对极坐标网格上的黄斑扇形区分析显示，GCLP的VD与NFL、GCIPL和黄斑神经节细胞复合体（GCC）厚度相关（R2分别为0.26、0.15和0.73，所有P<0.01）。GCC的相关性明显更强。
我们在PR-OCTA上定义了黄斑区的解剖学GCLP层板，即联合GCIPL的前75%。其密度与GCC的相关性最佳，GCC也包含NFL，这表明它至少也为NFL的后部供血。
绘制黄斑GCLP有望用于评估灌注，特别是在青光眼和视神经病变等疾病中。
