Tai Shih-Huang, Hsu Hao-Hsiang, Huang Sheng-Yang, Chen Yu-Ning, Chen Liang-Yi, Lee Ai-Hua, Lee Ai-Chiang, Lee E-Jian
Neurophysiology Laboratory, Department of Surgery, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, 138 Sheng-Li Road, Tainan, 70428, Taiwan.
Department of Occupational Safety and Health, Chung Hwa University of Medical Technology, Tainan, Taiwan.
J Neuroimmune Pharmacol. 2025 Jun 2;20(1):62. doi: 10.1007/s11481-025-10222-5.
In this study, we investigated the immunomodulatory effects of melatonin on B-cell maturation and its potential to mitigate immunodeficiency following transient focal cerebral ischemia in mice. Transient middle cerebral artery occlusion (MCAO) was induced in adult C57BL/6 mice, followed by melatonin administration. We evaluated the effects of melatonin on neutrophil infiltration, macrophage and microglial activation in the ischemic brain, and B-cell maturation in both the central nervous system and peripheral blood. Melatonin treatment significantly reduced pro-inflammatory cell infiltration in the ischemic hemisphere and promoted B-cell maturation, reflecting robust immunomodulatory activity. These immune changes coincided with improved neurological outcomes and reduced cerebral edema. Collectively, our findings suggest that melatonin may serve as a promising therapeutic candidate in ischemic stroke, addressing both immunodeficiency and neuronal injury.
在本研究中，我们调查了褪黑素对B细胞成熟的免疫调节作用及其减轻小鼠短暂性局灶性脑缺血后免疫缺陷的潜力。在成年C57BL/6小鼠中诱导短暂性大脑中动脉闭塞（MCAO），随后给予褪黑素。我们评估了褪黑素对缺血性脑内中性粒细胞浸润、巨噬细胞和小胶质细胞活化以及中枢神经系统和外周血中B细胞成熟的影响。褪黑素治疗显著减少了缺血半球的促炎细胞浸润并促进了B细胞成熟，反映出强大的免疫调节活性。这些免疫变化与改善的神经学结果和减轻的脑水肿相一致。总体而言，我们的研究结果表明，褪黑素可能是缺血性中风中有前景的治疗候选药物，可解决免疫缺陷和神经元损伤问题。
