Qin Xinyu, Guo Zhihong, Nie Zhangyao, Yang Yizhou, Zhang Ruiqi, Wang Xianbing, Zhang Zongying, Han Chenggui, Wang Ying
State Key Laboratory of Agricultural And Forestry Biosecurity and Ministry of Agriculture Key Laboratory of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing, 100193, China.
State Key Laboratory of Plant Environmental Resilience, College of Biological Sciences, China Agricultural University, Beijing, 100193, China.
Arch Virol. 2025 Jun 7;170(7):151. doi: 10.1007/s00705-025-06344-z.
A novel tri-segmented virus, tentatively named "Chenopodium trirhavirus 1" (CheTRV1), was identified in Chenopodium album in China using high-throughput sequencing combined with conventional Sanger sequencing after RT-PCR. The genome of CheTRV1 consists of three negative-sense single-stranded RNAs designated as RNA1, RNA2, and RNA3. RNAs 1-3 of CheTRV1 share 57.93-64.41%, 48.16-56.96%, and 38.12-42.22% nucleotide sequence identity with the corresponding segments of previously reported trirhaviruses. The 3'- and 5'-termini of the three segments are similar and exhibit partial inverse complementarity. The ORFs of CheTRV1 are separated by a conserved gene junction region with the consensus motif 3'-AAUUCUUUUGN(N)UUC- 5'. RNA1 consists of 6749 nucleotides (nt) and encodes a single putative L protein. RNA2 is 4393 nt in length and contains four genes in the order 3'-N-P2-P3-P4-5'. The smaller RNA3 consists of 3897 nt and has three genes in the order 3'-P6-P7-P8-5'. Phylogenetic analysis and pairwise comparisons suggested that CheTRV1 is most closely related to Medicago trirhavirus 1, with 68.82% amino acid sequence identity in the L protein. This is the first report of the complete genome sequence of a tri-segmented rhabdovirus, and it expands our knowledge of rhabdovirus evolution.
在中国，通过高通量测序结合逆转录聚合酶链反应（RT-PCR）后的传统桑格测序，在藜中鉴定出一种新型的三段式病毒，暂命名为“藜三havirus 1”（CheTRV1）。CheTRV1的基因组由三个负义单链RNA组成，分别命名为RNA1、RNA2和RNA3。CheTRV1的RNA 1-3与先前报道的三haviruses的相应片段的核苷酸序列同一性分别为57.93-64.41%、48.16-56.96%和38.12-42.22%。这三个片段的3'和5'末端相似，并表现出部分反向互补性。CheTRV1的开放阅读框由一个保守的基因连接区隔开，其共有基序为3'-AAUUCUUUUGN(N)UUC-5'。RNA1由6749个核苷酸（nt）组成，编码一个单一的假定L蛋白。RNA2长度为4393 nt，按3'-N-P2-P3-P4-5'的顺序包含四个基因。较小的RNA3由3897 nt组成，按3'-P6-P7-P8-5'的顺序有三个基因。系统发育分析和成对比较表明，CheTRV1与苜蓿三havirus 1关系最为密切，L蛋白的氨基酸序列同一性为68.82%。这是关于三段式弹状病毒完整基因组序列的首次报道，它扩展了我们对弹状病毒进化的认识。
