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大鼠大脑皮层中钙和钙调蛋白依赖性肌球蛋白轻链磷酸化活性的亚细胞分布

Subcellular distribution of calcium- and calmodulin-dependent myosin light chain phosphorylating activity in rat cerebral cortex.

作者信息

Edelman A M, Hunter D D, Hendrickson A E, Krebs E G

出版信息

J Neurosci. 1985 Oct;5(10):2609-17. doi: 10.1523/JNEUROSCI.05-10-02609.1985.

DOI:10.1523/JNEUROSCI.05-10-02609.1985
PMID:4045548
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6565136/
Abstract

The subcellular distribution of Ca2+- and calmodulin-dependent myosin light chain phosphorylating activity in rat cerebral cortex was studied. The activity showed a high degree of association to nerve endings (either crude or purified by discontinuous sucrose density gradient centrifugation). After osmotic shock of the nerve endings, the activity was largely membrane associated and could not be released from membranes by freeze-thawing, dilution, 75 mM NaCl or 4 mM EDTA. The association of Ca2+/calmodulin-dependent myosin light chain phosphorylating activity with synaptosomal membranes suggests a role for calcium-dependent myosin phosphorylation in events relating to neurotransmission.

摘要

研究了大鼠大脑皮层中钙和钙调蛋白依赖性肌球蛋白轻链磷酸化活性的亚细胞分布。该活性与神经末梢（无论是粗提物还是通过不连续蔗糖密度梯度离心纯化的神经末梢）高度相关。对神经末梢进行渗透休克后，该活性主要与膜相关，并且不能通过冻融、稀释、75 mM氯化钠或4 mM乙二胺四乙酸从膜中释放出来。钙/钙调蛋白依赖性肌球蛋白轻链磷酸化活性与突触体膜的结合表明钙依赖性肌球蛋白磷酸化在与神经传递相关的事件中起作用。

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