Kaplán P, Racay P, Lehotský J, Mézesová V
Comenius University, Jessenius Medical Faculty, Department of Biochemistry, Martin, Slovak Republic.
Neurochem Res. 1995 Jul;20(7):815-20. doi: 10.1007/BF00969693.
Effect of various oxygen free radical generating systems and an oxidant H2O2 on brain endoplasmic reticulum (ER) membrane fluidity was examined using fluorescent membrane probe 1,6-diphenyl-1,3,5-hexatriene, DPH. The relative potency of free radical generating systems to decrease membrane fluidity increased in this order: FeCl3-EDTA, FeSO4-EDTA, FeSO4-EDTA/hydrogen peroxide. Potency to decrease membrane fluidity correlated well with these systems' potencies to induce lipid peroxidation, as detected by conjugated diene formation. Treatment of ER membranes with H2O2 had no effect on fluidity or conjugated diene formation. Using the two most potent free radical generating systems, FeSO4-EDTA and FeSO4-EDTA/hydrogen peroxide, a protective effect of the novel antihypoxic and antiarrhytmic drug stobadine was tested. Stobadine and two well-known antioxidants, alpha-tocopherol acetate and butylated hydroxytoluene, demonstrated the ability to prevent free radical induced alterations in ER membrane fluidity. These results provide new evidence of stobadine's protective effect on membranes attacked by oxygen free radicals.
使用荧光膜探针1,6 - 二苯基 - 1,3,5 - 己三烯（DPH）检测了各种氧自由基生成系统和氧化剂过氧化氢（H₂O₂）对脑内质网（ER）膜流动性的影响。自由基生成系统降低膜流动性的相对效力按以下顺序增加：FeCl₃ - EDTA、FeSO₄ - EDTA、FeSO₄ - EDTA/过氧化氢。降低膜流动性的效力与这些系统诱导脂质过氧化的效力密切相关，脂质过氧化通过共轭二烯的形成来检测。用过氧化氢处理内质网膜对膜流动性或共轭二烯的形成没有影响。使用两种最有效的自由基生成系统FeSO₄ - EDTA和FeSO₄ - EDTA/过氧化氢，测试了新型抗缺氧和抗心律失常药物司他定的保护作用。司他定以及两种著名的抗氧化剂醋酸α - 生育酚和丁基化羟基甲苯，均表现出防止自由基诱导的内质网膜流动性改变的能力。这些结果为司他定对受氧自由基攻击的膜的保护作用提供了新证据。
