Adzick N S, Harrison M R, Glick P L, Beckstead J H, Villa R L, Scheuenstuhl H, Goodson W H
J Pediatr Surg. 1985 Aug;20(4):315-9. doi: 10.1016/s0022-3468(85)80210-4.
We compared simultaneous healing processes in fetal, newborn, and maternal rabbits using a miniaturized wound cylinder of expanded Gore-Tex tubing. The tubing was placed subcutaneously in fetal and maternal rabbits on day 23 of pregnancy (term = 31 to 32 days), and in 7-day-old newborn rabbits. At specific intervals, the tubing was removed and analyzed for hydroxyproline accumulation, histology, and cellular enzyme-histochemistry. Granulation tissue ingrowth and accumulation of hydroxyproline were each inversely related to age (fetus greater than newborn greater than maternal). The fetus showed an impressive infiltration of macrophages by day 4, fibroblasts by day 7, and a conspicuous lack of neutrophils in all specimens. Newborns and mothers had few cells until day 7, when a mixture of macrophages, neutrophils, and some fibroblasts appeared. We conclude that the fetus heals wounds rapidly by both mesenchymal cell proliferation and collagen deposition, and that these processes are more rapid in fetuses than in newborn or adult animals despite relative fetal hypoxemia.
我们使用微型扩张 Gore-Tex 管制成的伤口柱体，比较了胎儿、新生兔和母兔的同步愈合过程。在妊娠第23天（孕期为31至32天），将该管皮下植入胎儿和母兔体内，以及7日龄新生兔体内。在特定时间间隔，取出该管并分析羟脯氨酸积累、组织学和细胞酶组织化学情况。肉芽组织向内生长和羟脯氨酸积累均与年龄呈负相关（胎儿＞新生兔＞母兔）。胎儿在第4天可见大量巨噬细胞浸润，第7天可见成纤维细胞，且所有标本中明显缺乏中性粒细胞。新生兔和母兔在第7天之前细胞很少，之后出现巨噬细胞、中性粒细胞和一些成纤维细胞的混合细胞群。我们得出结论，胎儿通过间充质细胞增殖和胶原蛋白沉积快速愈合伤口，尽管胎儿存在相对低氧血症，但这些过程在胎儿中比在新生或成年动物中更快。
