Fathollahi Qarachoboogh Ayoub, Alijanpour Ahmad, Hosseini Bahman, Banj Shafiei Abbas
Department of Forestry, Faculty of Natural Resources, Urmia University, Urmia, Iran.
Department of Horticultural, Faculty of Agriculture, Urmia University, Urmia, Iran.
Sci Rep. 2025 Jun 2;15(1):19243. doi: 10.1038/s41598-025-03073-w.
In order to propagate and regenerate the Juniperus excelsa L. in large quantities, this research was conducted to identify the appropriate mineral salt concentrations and plant growth regulators toward forest ecosystem sustainability. Different basal culture media including Olive Medium (OM), Murashige and Skoog (MS), Driver and Kuniyuki (DKW), Woody Plant Medium (WPM), ½ MS, and ¼ MS supplemented with 1 mg L 6-benzylaminopurine (BAP) and 0.1 mg L indole-3-acetic acid (IAA) as plant growth regulators (PGR) were examined on the explants' aerial organ growth, rooting and shoot proliferation. The experiment was carried out in a completely randomized design with three replicates and each replicate consisted of six samples. The results showed that the OM, DKW, and WPM culture media achieved the highest juniper regeneration of 100% when combined with BAP and IAA. The basic culture medium DKW produced the highest average number of shoots (8.05 shoots/explant) in the presence of 1 mg L BAP and 0.1 mg L IAA, however the highest shoot length (9.6 mm) observed in the DKW culture medium with no PGR. It is concluded that DKW and OM were the proper and most efficient culture medium for J. excelsa branch propagation and rooting, respectively.
为了大量繁殖和再生卓越刺柏（Juniperus excelsa L.），开展本研究以确定适合森林生态系统可持续性的矿质盐浓度和植物生长调节剂。在添加1毫克/升6-苄基腺嘌呤（BAP）和0.1毫克/升吲哚-3-乙酸（IAA）作为植物生长调节剂（PGR）的情况下，对包括橄榄培养基（OM）、Murashige和Skoog培养基（MS）、Driver和Kuniyuki培养基（DKW）、木本植物培养基（WPM）、1/2MS和1/4MS在内的不同基础培养基进行外植体地上器官生长、生根和芽增殖的研究。试验采用完全随机设计，重复3次，每次重复由6个样本组成。结果表明，当与BAP和IAA组合时，OM、DKW和WPM培养基的刺柏再生率最高，达到100%。在添加1毫克/升BAP和0.1毫克/升IAA的情况下，基础培养基DKW产生的平均芽数最高（8.05个芽/外植体），然而在未添加PGR的DKW培养基中观察到最高的芽长（9.6毫米）。得出的结论是，DKW和OM分别是卓越刺柏枝条繁殖和生根的合适且最有效的培养基。
