Ahmed Habiba A, El-Maradny Yousra A, Shalaby Manal A, El-Menshawy Hany, Abd El-Wahab Abeer E
Plant Biochemistry Department, National Research Centre, Dokki, 12622, Giza, Egypt.
Medical Biotechnology Department, Institute of Genetic Engineering and Biotechnology, City of Scientific Research and Technological Applications (SRTA-City), New Borg EL-Arab, 21934, Alexandria, Egypt.
Sci Rep. 2025 Jun 2;15(1):19263. doi: 10.1038/s41598-025-03355-3.
Microbial biofilms present a significant global health challenge, as they are associated with severe chronic infections and the emergence of antibiotic resistance. Currently, only a limited number of clinically available drugs effectively target microbial biofilms. This underscores the urgent need for the development of new sustainable therapeutic strategies to address biofilm-associated infections. Developing a sustainable and biodegradable preparation for eradicating microbial biofilms is critically important. In this study, chitosan was extracted from shrimp shell waste and utilized to prepare salicylic acid-loaded chitosan nanoparticles (NPs) using various synthesis methods. The particle size of the prepared nanoparticles ranged from 287.4 to 226.3 nm, with zeta potential values between + 36.6 and + 41.3 mV, indicating good stability. The nanoparticles demonstrated safety, with half maximal inhibitory concentration (IC) values ranging from 1009 to 1346 µg/mL. The combination of chitosan and salicylic acid exhibited significant antibiofilm activity against Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, and Candida albicans, with particularly high efficacy against Candida albicans, achieving up to 85% biofilm inhibition. While the particle size and antibiofilm activity of the nanoforms showed minimal differences, formulation M4, using sodium alginate, stands out as the most eco-friendly option. This study highlights the potential of bio-sustainable chitosan-based formulations for combating biofilm formation and addressing antimicrobial resistance.
微生物生物膜是全球健康面临的重大挑战，因为它们与严重的慢性感染和抗生素耐药性的出现有关。目前，临床上可用的有效靶向微生物生物膜的药物数量有限。这凸显了开发新的可持续治疗策略以应对生物膜相关感染的迫切需求。开发一种可持续且可生物降解的制剂来根除微生物生物膜至关重要。在本研究中，从虾壳废料中提取壳聚糖，并采用各种合成方法将其用于制备负载水杨酸的壳聚糖纳米颗粒（NPs）。制备的纳米颗粒粒径范围为287.4至226.3 nm，zeta电位值在+36.6至+41.3 mV之间，表明稳定性良好。纳米颗粒显示出安全性，半数最大抑制浓度（IC）值范围为1009至1346 μg/mL。壳聚糖和水杨酸的组合对大肠杆菌、肺炎克雷伯菌、金黄色葡萄球菌和白色念珠菌表现出显著的抗生物膜活性，对白色念珠菌的疗效尤其高，生物膜抑制率高达85%。虽然纳米制剂的粒径和抗生物膜活性差异最小，但使用海藻酸钠的制剂M4是最环保的选择。本研究突出了基于壳聚糖的生物可持续制剂在对抗生物膜形成和解决抗菌耐药性方面的潜力。
