Khan Fazlurrahman, Oh Dokyung, Chandika Pathum, Jo Du-Min, Bamunarachchi Nilushi Indika, Jung Won-Kyo, Kim Young-Mog
Research Center for Marine Integrated Bionics Technology, Pukyong National University, Busan 48513, Republic of Korea.
Department of Food Science and Technology, Pukyong National University, Busan 48513, Republic of Korea.
Colloids Surf B Biointerfaces. 2022 Mar;211:112307. doi: 10.1016/j.colsurfb.2021.112307. Epub 2021 Dec 24.
Phloroglucinol (PG) was encapsulated into chitosan nanoparticles (CSNPs) using a simple ionic gelification technique, and the inhibitory activity of the resulting nanoparticles on microbial mono- and dual-species biofilms was investigated. PG-CSNPs were determined to be spherical with a rough surface, and had an average diameter and zeta potential of 414.0 ± 48.5 nm and 21.1 ± 1.2 mV, respectively. The rate of PG release from the loaded CSNPs was found to increase in acidic environment. The loading capacity and encapsulation efficiency of PG to CSNPs were determined to be 18.74% and 22.4%, respectively. The prepared PG-CSNPs exhibited inhibitory effects on mono-species biofilms such as Candida albicans, Staphylococcus aureus, Klebsiella pneumoniae, Streptococcus mutans, and dual-species such as C. albicans-K. pneumoniae/S. aureus/S. mutans. The PG-CSNPs were found to be more effective in inhibiting and eradicating mono- and dual-species biofilms than pure PG. In addition, PG-CSNPs were found to enhance the efficacy of several antimicrobial drugs against mature mono- and dual-species biofilms. This work demonstrates that PG-CSNPs may provide an alternative method for treating infections caused by biofilm-forming pathogens.
采用简单的离子凝胶化技术将间苯三酚（PG）包裹于壳聚糖纳米颗粒（CSNPs）中，并研究所得纳米颗粒对微生物单菌种和双菌种生物膜的抑制活性。PG-CSNPs被确定为表面粗糙的球形，平均直径和zeta电位分别为414.0±48.5 nm和21.1±1.2 mV。发现负载的CSNPs中PG在酸性环境下的释放速率增加。PG对CSNPs的负载量和包封率分别确定为18.74%和22.4%。制备的PG-CSNPs对白色念珠菌、金黄色葡萄球菌、肺炎克雷伯菌、变形链球菌等单菌种生物膜以及白色念珠菌-肺炎克雷伯菌/金黄色葡萄球菌/变形链球菌等双菌种生物膜均表现出抑制作用。发现PG-CSNPs在抑制和根除单菌种和双菌种生物膜方面比纯PG更有效。此外，还发现PG-CSNPs可增强几种抗菌药物对成熟单菌种和双菌种生物膜的疗效。这项工作表明，PG-CSNPs可能为治疗由形成生物膜的病原体引起的感染提供一种替代方法。
