Lambert Camille L G, van Mierlo Guido, Bues Johannes J, Guillaume-Gentil Orane J, Deplancke Bart
Laboratory of Systems Biology and Genetics, Institute of Bioengineering, School of Life Sciences, Swiss Federal Institute of Technology (EPFL), Lausanne, Switzerland.
Nat Genet. 2025 Jun 2. doi: 10.1038/s41588-025-02198-y.
The completion of the Human Genome Project catalyzed the development of 'omics' technologies, enabling the detailed exploration of biological systems at an unprecedented molecular scale. Microfluidics has transformed the omics toolbox by facilitating large-scale, high-throughput and highly accurate measurements of DNA and RNA, driving the transition from bulk to single-cell analyses. This transition has ushered in a new era, moving beyond a gene- and protein-centric perspective toward a holistic understanding of cellular phenotypes. This emerging 'single-cell phenomics era' integrates diverse omics datasets with spatial, morphological and temporal phenotypes to provide a comprehensive perspective on cellular function. This Review highlights how microfluidics addressed key challenges in the transition to single-cell omics and explores how lessons learned from these efforts will propel the single-cell phenomics revolution. Furthermore, we discuss emerging opportunities in which integrative single-cell phenomics could serve as a foundation for transformative discoveries in biology.
人类基因组计划的完成推动了“组学”技术的发展，使得在前所未有的分子尺度上对生物系统进行详细探索成为可能。微流控技术通过促进对DNA和RNA的大规模、高通量及高精度测量，改变了组学工具箱，推动了从整体分析到单细胞分析的转变。这一转变开启了一个新时代，从以基因和蛋白质为中心的视角，迈向对细胞表型的全面理解。这个新兴的“单细胞表型组学时代”将多样的组学数据集与空间、形态和时间表型整合在一起，以提供关于细胞功能的全面视角。本综述重点介绍了微流控技术如何应对向单细胞组学转变过程中的关键挑战，并探讨了从这些努力中汲取的经验教训将如何推动单细胞表型组学革命。此外，我们还讨论了新兴机遇，即整合的单细胞表型组学可作为生物学变革性发现的基础。
