Dourado Fernandes Clara, Grünewald Alina, Hadzhieva Zoya, Oechsler Bruno F, Sayer Claudia, Hermes de Araújo Pedro H, Boccaccini Aldo R
Department of Chemical Engineering and Food Engineering, Federal University of Santa Catarina, 88040-900 Florianópolis, Santa Catarina, Brazil.
Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Cauerstr. 6, 91058 Erlangen, Germany.
ACS Appl Bio Mater. 2025 Jun 16;8(6):4791-4804. doi: 10.1021/acsabm.5c00176. Epub 2025 Jun 3.
As human longevity increases, the prevalence of age-related pathologies grows, driving the need for advances in regenerative medicine. This research evaluates poly(propylene succinate--glycerol succinate) (PPSG) as a renewable additive in electrospun polycaprolactone (PCL) mats, to develop biodegradable and biocompatible scaffolds incorporating 45S5 bioactive glass (BG) particles of size ∼4 μm. Electrospinning solutions with 20% (w/v) acetic acid were used, with PPSG proportions of 5%, 10%, and 15% by weight. Additionally, BG particles were incorporated at 5, 15, and 30 wt % to enhance bioactivity. Uniform fibers were achieved with 10% PPSG at 0.4 mL/h and 15 kV, yielding bead-free structures. PPSG increased fiber diameter and mechanical properties, with Young's modulus (E) rising from 1.7 ± 1 MPa (pure PCL) to 8.8 ± 1.5 MPa (PCL/20PPSG). Ultimate tensile strength (Σ) improved from 0.4 MPa (PCL) to 1.5 MPa (PCL/10PPSG). BG incorporation enhanced bioactivity but reduced mechanical stability due to particle distribution. Samples containing 15% BG exhibited significantly increased NHDF cell viability, and hydrophilicity improved with PPSG (reduced from 110° to 28 ± 3°). Biodegradability testing revealed a 45% ± 5 mass loss for 10% PPSG fibers over 35 days. The PCL/PPSG/BG composite demonstrates enhanced mechanical strength, bioactivity, and cell viability, making it a promising candidate for soft tissue engineering and regenerative medicine.
随着人类寿命的延长，与年龄相关的疾病患病率不断上升，这推动了再生医学发展的需求。本研究评估了聚（琥珀酸丙酯 - 琥珀酸甘油酯）（PPSG）作为电纺聚己内酯（PCL）垫中的可再生添加剂，以开发包含尺寸约为4μm的45S5生物活性玻璃（BG）颗粒的可生物降解且生物相容的支架。使用含20%（w/v）乙酸的电纺溶液，PPSG的重量比例为5%、10%和15%。此外，以5、15和30 wt%的比例加入BG颗粒以增强生物活性。在0.4 mL/h和15 kV的条件下，10%的PPSG实现了均匀的纤维，产生无珠结构。PPSG增加了纤维直径和机械性能，杨氏模量（E）从1.7±1 MPa（纯PCL）提高到8.8±1.5 MPa（PCL/20PPSG）。极限拉伸强度（Σ）从0.4 MPa（PCL）提高到1.5 MPa（PCL/10PPSG）。BG的加入增强了生物活性，但由于颗粒分布导致机械稳定性降低。含有15% BG的样品显示NHDF细胞活力显著增加，并且随着PPSG的加入亲水性得到改善（从110°降至28±3°）。生物降解性测试表明，10% PPSG纤维在35天内质量损失45%±5。PCL/PPSG/BG复合材料表现出增强的机械强度、生物活性和细胞活力，使其成为软组织工程和再生医学的有前途的候选材料。
