Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058 Erlangen, Germany.
Institute of Biomedical Engineering, Bogazici University, Rasathane St., Kandilli 34684, Istanbul, Turkey.
Biomed Mater. 2023 Sep 1;18(5). doi: 10.1088/1748-605X/acf0ad.
Aligned biodegradable fibers incorporating bioactive glass particles are being highly investigated for tissue engineering applications. In this study, 5, 7 and 10 wt% melt-derived 1393B3 borate glass (BG) microparticles (average size: 3.15 µm) were incorporated in 83 wt% polycaprolactone (PCL) and 17 wt% gelatin (GEL) (83PCL/17GEL) solutions to produce aligned electrospun composite nanofiber mats. Addition of 5 wt% BG particles significantly increased the alignment of the nanofibers. However, further incorporation of BG particles led to reduced degree of alignment, likely due to an increase of viscosity. Mechanical tests indicated a tensile modulus and tensile strength of approximately 51 MPa and 3.4 MPa, respectively, for 5 wt% addition of 1393B3 BG microparticles, values considered suitable for soft tissue engineering applications. However, with the increasing amount of 1393B3 BG, the nanofiber mats became brittle. Contact angle was reduced after the addition of 5 wt% of 1393B3 BG particles from45° to39°. Cell culture studies with normal human dermal fibroblast (NHDF) cells indicated that 5 wt% 1393B3 BG incorporated nanofiber mats were cytocompatible whereas higher doping with 1393B3 BGs reduced biocompatibility. Overall, 5 wt% 1393B3 BG doped PCL/GEL nanofiber mats were aligned with high biocompatibility exhibiting desirable mechanical properties for soft tissue engineering, which indicates their potential for applications requiring aligned nanofibers, such as peripheral neural regeneration.
用于组织工程应用的掺入生物活性玻璃颗粒的可生物降解纤维正在被深入研究。在这项研究中，将 5wt%、7wt%和 10wt%熔融衍生的 1393B3 硼酸盐玻璃（BG）微球（平均尺寸：3.15µm）掺入 83wt%聚己内酯（PCL）和 17wt%明胶（GEL）（83PCL/17GEL）溶液中，以生产取向的电纺复合纳米纤维垫。添加 5wt%BG 颗粒可显著提高纳米纤维的取向度。然而，进一步加入 BG 颗粒会导致取向度降低，这可能是由于粘度增加所致。力学测试表明，加入 5wt%1393B3BG 微球后，拉伸模量和拉伸强度分别约为 51MPa 和 3.4MPa，这些值被认为适用于软组织工程应用。然而，随着 1393B3BG 含量的增加，纳米纤维垫变得易碎。加入 5wt%的 1393B3BG 后，接触角从 45°降低到 39°。用正常人皮肤成纤维细胞（NHDF）进行细胞培养研究表明，加入 5wt%的 1393B3BG 纳米纤维垫具有细胞相容性，而加入更高剂量的 1393B3BG 则降低了生物相容性。总体而言，5wt%的 1393B3BG 掺杂的 PCL/GEL 纳米纤维垫具有高生物相容性的取向性，表现出理想的机械性能，适用于软组织工程，这表明它们在需要取向纳米纤维的应用中具有潜力，例如周围神经再生。
