Mavis Merdiye, Serakinci Nedime
Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Near East University, Nicosia, Northern Cyprus.
Faculty of Arts and Sciences, Cyprus International University, Nicosia, Northern Cyprus.
Methods Mol Biol. 2025;2960:1-8. doi: 10.1007/7651_2025_631.
Mesenchymal stem cells (MSCs) have various characteristic properties such as self-renewal and multilineage differentiation capability, making them promising tools to be used in clinics in therapeutic settings as a cell-based therapy. For MSCs to be used for therapeutic applications, their prolonged expansion in culture is required to provide a sufficient number of cells. During the expansion in culture, MSCs experience only a restricted number of population doublings, and then they enter senescence. Becoming senescent impairs MSCs' proliferation capability, colony-forming efficacy, and ability to differentiate into various lineages and these limit their application in clinics. Thus, monitoring the senescence and aging of MSCs is essential for their use in therapeutic applications. In this chapter, we summarized the protocols to assess the aging of MSCs through senescence-associated β-galactosidase and colony-forming unit assay.
间充质干细胞（MSCs）具有多种特性，如自我更新和多向分化能力，这使其成为细胞疗法中有望应用于临床治疗的工具。为了将MSCs用于治疗应用，需要在培养中进行长时间扩增以提供足够数量的细胞。在培养扩增过程中，MSCs仅经历有限次数的群体倍增，然后进入衰老状态。进入衰老状态会损害MSCs的增殖能力、集落形成效率以及分化为各种谱系的能力，这些都限制了它们在临床上的应用。因此，监测MSCs的衰老对于其在治疗应用中的使用至关重要。在本章中，我们总结了通过衰老相关β-半乳糖苷酶和集落形成单位测定来评估MSCs衰老的方案。
