Sezgin Aydan, Emniyet Sert Asiye Aslı, Take Kaplanoğlu Gülnur, Karakaya Cengiz, Demirdağ Erhan, Cevher Akdulum M Funda, Erdem Mehmet, Erdem Ahmet, Öktem Mesut
Ankara Etlik City Hospital, Clinic of Obstetrics and Gynecology, Ankara, Türkiye.
Hitit University Faculty of Medicine, Department of Histology and Embryology, Çorum, Türkiye.
Turk J Obstet Gynecol. 2025 Jun 4;22(2):154-162. doi: 10.4274/tjod.galenos.2025.30161.
Evaluating the therapeutic effect of detorsion, resveratrol, and nifedipine on ovarian viability assessed by biochemical, histopathological and immunohistochemical parameters and markers of oxidative stress.
Twenty-four Sprague-Dawley rats were included in 4 groups, namely: sham operation, ischemia-reperfusion (I/R), I/R+10 mg/kg nifedipine (NIF), I/R+100 mg/kg resveratrol (RSV). In the study groups, bilateral 720º ovarian torsion was performed and continued for 3 hours, followed by detorsion for another 3 hours. Thirty minutes before the detorsion, NIF and RSV groups received respective treatments. Adnexectomy was performed, and evaluations were made for the expression of anti-müllerian hormone (AMH), vascular endothelial growth factor receptor 2 (VEGFR-2), markers of oxidative stress, and follicle counts. Blood AMH levels were measured.
No change in AMH levels was detected. Although the expression of AMH was significantly reduced following I/R alone, it remained similar to the control group in the NIF group. Meanwhile, the RSV group exhibited slightly lower expression than the control, although it was still higher than that observed with the I/R injury group. VEGFR-2 staining was similar in the I/R and NIF groups, but reduced in the RSV group. Markers of oxidative stress were similar between groups. Primordial follicle count was lower in the untreated I/R injury group compared to the control group (p<0.05). The NIF group had more secondary follicles than the I/R injury and RSV groups (p<0.05).
Nifedipine and resveratrol treatments did not influence AMH levels and antioxidant-oxidant system parameters in rats exposed to I/R injury. However, nifedipine had a positive effect on AMH expression and secondary follicle count, and resveratrol decreased the expression of VEGFR-2 in tissues, which can be an indicator of their clinical potential.
通过生化、组织病理学和免疫组化参数以及氧化应激标志物评估去扭转、白藜芦醇和硝苯地平对卵巢活力的治疗效果。
将24只Sprague-Dawley大鼠分为4组，即：假手术组、缺血再灌注（I/R）组、I/R + 10 mg/kg硝苯地平（NIF）组、I/R + 100 mg/kg白藜芦醇（RSV）组。在研究组中，进行双侧720°卵巢扭转并持续3小时，随后再进行3小时的去扭转。去扭转前30分钟，NIF组和RSV组接受相应治疗。进行附件切除术，并对抗苗勒管激素（AMH）、血管内皮生长因子受体2（VEGFR-2）的表达、氧化应激标志物和卵泡计数进行评估。检测血液中AMH水平。
未检测到AMH水平的变化。虽然单独I/R后AMH的表达显著降低，但在NIF组中仍与对照组相似。同时，RSV组的表达略低于对照组，尽管仍高于I/R损伤组。I/R组和NIF组的VEGFR-2染色相似，但RSV组降低。各组之间氧化应激标志物相似。未治疗的I/R损伤组的原始卵泡计数低于对照组（p<0.05）。NIF组的次级卵泡比I/R损伤组和RSV组更多（p<0.05）。
硝苯地平和白藜芦醇治疗对遭受I/R损伤的大鼠的AMH水平和抗氧化 - 氧化系统参数没有影响。然而，硝苯地平对AMH表达和次级卵泡计数有积极作用，白藜芦醇降低了组织中VEGFR-2的表达，这可能是它们临床潜力的一个指标。
