Effect of continuous chelation irrigation on transforming growth factor-β1 release: An assay.

CONTEXT

Root canal irrigants and medicaments influence the growth factors released from dentin and create a conducive environment for regeneration.

AIM

This study assesses the transforming growth factor-beta 1 (TGF-β1) release after using clodronate and etidronate as continuous chelating agents with two different medicaments calcium hydroxide (CH) and triple antibiotic paste (TAP).

SETTINGS AND DESIGN

This study involves an study.

MATERIALS AND METHODS

Ninety standard-sized dentin cylinders with apical sizes of 1 mm and 12 mm length were made from freshly extracted single-rooted premolars. Samples were randomly divided (45 each) and subjected to two intracanal medicaments, CH (Groups 1, 2, and 3) and TAP (Groups 4, 5, and 6). Three different irrigation protocols were followed: Group 1 and 4: Sequential use of 1.5% sodium hypochlorite (NaOCl) followed by 17% ethylenediaminetetraacetic acid (EDTA); Group 2 and 5: Etidronate + 1.5% NaOCl mixture; Group 3 and 6: 7.6% clodronate + 1.5% NaOCl mixture. The samples were stored in phosphate-buffered saline and subjected to enzyme-linked immunosorbent assay for TGF-β1quantification.

STATISTICAL ANALYSIS USED

One-way analysis of variance and Tukey's test with significance value <0.05.

RESULTS

Growth factor release was significantly higher in the clodronate irrigation groups irrespective of the intracanal medicament used ( < 0.05). There was no significant difference in TGF-β1 release when comparing the two intracanal medicaments among all three irrigation groups.

CONCLUSIONS

In the context of growth factor release, etidronate, and clodronate, which are regarded as substitutes for EDTA in regenerative endodontic procedures, performed equivalent to the gold standard irrigant, regardless of the medicament used.