Bastos Aline Costa, Gomes Maurício Ferreira, Pinheiro W B S, Botelho Anderson de Santana, Batista Raimundo Junior da Rocha, do Amarante Cristine Bastos, Rezende Taia Maria Berto, da Silva Yasmin Cunha, Antonio Ananda da Silva, Pereira Henrique Marcelo Gualberto, da Veiga Júnior Valdir Florêncio, Khayat André Salim, Yamada Elizabeth S, Bastos Gilmara de Nazareth Tavares
Laboratory of Neuroinflammation, Institute of Biological Sciences, Federal University of Pará, Belém, Brazil.
Oncology Research Center, Federal University of Pará, Belém, Brazil.
Front Pharmacol. 2025 May 20;16:1512570. doi: 10.3389/fphar.2025.1512570. eCollection 2025.
(Arruda) Schott () is commonly used by Amazonian riverine communities for the treatment of skin ulcers, although its effects as a wound healer have never been evaluated until now. Therefore, the wound-healing activity of the extracts from the stem and petiole of was investigated for the first time.
The extracts were characterized by chromatography coupled with spectroscopic or spectrometric methods (HPTLC-UV and UHPLC-MS), and free radical scavenging was verified using bioautography with the DPPH radical. Cytotoxicity was evaluated through the MTT method, and a scratch assay was employed to assess cell migration, while cell proliferation was evaluated through immunofluorescence for BrdU-positive cells.
Chemical characterization revealed the presence of 13 metabolites in ESML and EPML extracts. Analytical analysis of the extract demonstrated the elimination of free radicals by autobiography. The extracts did not demonstrate cytotoxicity in fibroblasts and cell migration and proliferation were, significantly, increased reducing the wound area .
Thus, it was observed that the extracts from the stem and petiole of possess potential wound-healing effects in fibroblasts . This is a pioneering study that provides insights for future studies on the mechanisms of action of this species, in addition to validating the ethnopharmacological knowledge of this species used in the Amazon.
（阿鲁达）肖特氏植物通常被亚马逊河沿岸社区用于治疗皮肤溃疡，尽管其作为伤口愈合剂的效果至今尚未得到评估。因此，首次对该植物茎和叶柄提取物的伤口愈合活性进行了研究。
提取物通过色谱结合光谱或光谱法（HPTLC-UV和UHPLC-MS）进行表征，并使用DPPH自由基生物自显影法验证自由基清除能力。通过MTT法评估细胞毒性，采用划痕试验评估细胞迁移，通过免疫荧光检测BrdU阳性细胞评估细胞增殖。
化学表征显示ESML和EPML提取物中存在13种代谢物。提取物的分析表明通过生物自显影法可清除自由基。提取物在成纤维细胞中未显示细胞毒性，并且细胞迁移和增殖显著增加，伤口面积减小。
因此，观察到该植物茎和叶柄的提取物对成纤维细胞具有潜在的伤口愈合作用。这是一项开创性研究，除了验证亚马逊地区使用的该物种的民族药理学知识外，还为该物种作用机制的未来研究提供了见解。
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