de Sousa Oliveira Daniele Santana, da Silva Elis Dionisio, Galvão Júnior Fábio Farias, Moura Danielle Maria Nascimento, Sales-Junior Policarpo Ademar, Brelaz-de-Castro Maria Carolina Accioly, Pereira Valéria Rêgo Alves
Postgraduation Program in Therapeutic Innovation, Federal University of Pernambuco, Recife, Pernambuco, Brazil.
Health and Biotechnology Institute, Federal University of Amazonas, Coari, Amazonas, Brazil.
Front Med (Lausanne). 2025 May 20;12:1591148. doi: 10.3389/fmed.2025.1591148. eCollection 2025.
Chagas disease is considered a public health issue, especially in Latin America. To this date, the course of the infection caused by the parasite is yet to be completely understood and the conventional treatment do not promote a cure in the chronic phase, meaning there is an urgent need to discover new drugs. The expression of reporter genes by transgenic parasites has become an important tool in the screening of new compounds, whether in the study of the parasite, in the development of and assays, or in the application of High-Throughput Screening utilizing compounds collections. This review sought to gather information about transgenic applications in screening studies of compounds with action specifically against Chagas disease, the reporter genes in use, besides the highlighted characteristics of each one by the literature, including the performed assays, evolutionary forms and techniques applied, aiming to facilitate the identification of the reporter gene system or research model whose characteristics best adapt to the needs of new studies, contributing to the decisions about a framework adaptable to the reality of laboratories, in the screening of potentially trypanocidal compounds.
恰加斯病被视为一个公共卫生问题，尤其是在拉丁美洲。迄今为止，由该寄生虫引起的感染病程尚未完全明了，传统治疗方法无法治愈慢性期感染，这意味着迫切需要发现新药物。转基因寄生虫表达报告基因已成为筛选新化合物的重要工具，无论是在寄生虫研究、检测方法的开发与应用中，还是在利用化合物库进行的高通量筛选中。本综述旨在收集有关转基因在筛选对恰加斯病有特效作用的化合物的研究中的应用信息、所使用的报告基因，以及文献中突出的每种报告基因的特征，包括所进行的检测、进化形式和应用的技术，旨在便于识别其特征最适合新研究需求的报告基因系统或研究模型，为筛选潜在杀锥虫化合物时有关适用于实验室实际情况的框架的决策提供帮助。
