Shen Lisha
Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, 117604, Singapore; Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, 117543, Singapore.
Trends Plant Sci. 2025 Jun;30(6):629-641. doi: 10.1016/j.tplants.2024.11.012. Epub 2024 Dec 19.
Epitranscriptomic regulation has emerged as a crucial layer of gene control where RNA modifications, particularly N-methyladenosine (mA), introduce complexity and versatility to gene regulation. Increasing evidence suggests that epitranscriptomic regulation through phase separation plays critical roles in mediating RNA metabolism during plant development and stress responses. mA-associated biomolecular condensates formed via phase separation act as dynamic cellular hotspots where mA effectors, RNAs, and other regulatory proteins coalesce to facilitate RNA regulation. Moreover, mA modulates condensate assembly. Herein, I summarize the current understanding of how mA- and mA effector-mediated formation of biomolecular condensates mediates plant development and stress adaptation. I also discuss several working models for mA-associated biomolecular condensates and highlight the prospects for future research on epitranscriptomic regulation through phase separation.
表观转录组调控已成为基因控制的关键层面，其中RNA修饰，特别是N6-甲基腺苷（m6A），为基因调控引入了复杂性和多样性。越来越多的证据表明，通过相分离进行的表观转录组调控在植物发育和应激反应过程中介导RNA代谢中起着关键作用。通过相分离形成的与m6A相关的生物分子凝聚物充当动态细胞热点，m6A效应物、RNA和其他调节蛋白在其中聚集以促进RNA调控。此外，m6A调节凝聚物组装。在此，我总结了目前对m6A和m6A效应物介导的生物分子凝聚物形成如何介导植物发育和应激适应的理解。我还讨论了与m6A相关的生物分子凝聚物的几种工作模型，并强调了通过相分离进行表观转录组调控的未来研究前景。
