Sena Layse de Góis, Meyer Maria Davoli, Ricardo Mariana Gallante, Araújo Isaac Jordão de Souza, Rontani Julia Puppin, Pecorari Vanessa Arias, Martinez Elizabeth Ferreira, Teixeira Lucas Novaes, Nociti-Junior Francisco Humberto, Lisboa-Filho Paulo Noronha, Kantovitz Kamila Rosamilia
Faculdade São Leopoldo Mandic, School of Dentistry, Campinas, SP, Brazil.
University of Saskatchewan, Dental School, Department of Operative Dentistry, Saskatoon, SK, Canada.
Braz Oral Res. 2025 Jun 2;39:e059. doi: 10.1590/1807-3107bor-2025.vol39.059. eCollection 2025.
The present in vitro study evaluated the effect of titanium dioxide nanotubes (nTiO2) incorporated into glass ionomer cement (GIC) on the growth and viability of Lactobacillus acidophilus (L. acidophilus). GIC (Ketac Molar EasyMix® = KM) was added to concentrations of 0%, 3%, 5%, 7% by weight of nTiO2 (20 nm in size). L. acidophilus strains (1x108 CFU/mL) were cultivated on GIC discs with or without the addition of nTiO2 for 1, 3 and 7 days, and the following parameters were evaluated: inhibition zone (mm) (n = 6); cell viability (Live/Dead) (n = 6); cell morphology (SEM) (15 KV, 2000X, n = 3). The data were submitted to ANOVA and the Tukey and Dunnett tests (α = 0.05). Regarding the agar diffusion test, there was no difference between GIC and the groups containing nTiO2 (p > 0.05). As for bacterial viability, the percentage of viable bacteria was lower for GIC+7% nTiO2 (p ≤ 0.05). There was no difference in the percentage of non-viable bacteria (p > 0.05). In addition, the morphology of L. acidophilus did not change in the presence of nTiO2. It can be concluded that the incorporation of titanium dioxide nanotubes into GIC, particularly at 5%, reduced L. acidophilus viability, and might hence interfere negatively with the initial colonization process of the bacterial biofilm.
本体外研究评估了掺入玻璃离子水门汀（GIC）中的二氧化钛纳米管（nTiO₂）对嗜酸乳杆菌（L. acidophilus）生长和活力的影响。将GIC（Ketac Molar EasyMix® = KM）添加到按重量计0%、3%、5%、7%的nTiO₂（尺寸为20 nm）浓度中。嗜酸乳杆菌菌株（1x10⁸ CFU/mL）在添加或不添加nTiO₂的GIC圆盘上培养1、3和7天，并评估以下参数：抑菌圈（mm）（n = 6）；细胞活力（活/死）（n = 6）；细胞形态（扫描电子显微镜）（15千伏，2000倍，n = 3）。数据进行方差分析以及Tukey和Dunnett检验（α = 0.05）。关于琼脂扩散试验，GIC与含nTiO₂的组之间没有差异（p > 0.05）。至于细菌活力，GIC + 7% nTiO₂的活菌百分比更低（p≤0.05）。非活菌百分比没有差异（p > 0.05）。此外，在nTiO₂存在的情况下嗜酸乳杆菌的形态没有变化。可以得出结论，将二氧化钛纳米管掺入GIC中，特别是在5%的浓度下，会降低嗜酸乳杆菌的活力，因此可能会对细菌生物膜的初始定植过程产生负面影响。
