四氟化钛、酪蛋白磷酸肽-无定形磷酸钙和氟化钠在体外预防辐照牛牙釉质和牙本质脱矿中的比较。

Comparison of TiF4, CPP-ACP, and NaF in preventing demineralization in irradiated bovine enamel and dentin in vitro.

作者信息

Yao Lin, Li Yanyao, Fu Di, Ji Mengzhen, Zou Ling, Jiang Li

机构信息

Sichuan University, West China Hospital of Stomatology, State Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases & Department of Conservative Dentistry and Endodontics, Chengdu, China.

Sichuan University, West China Hospital of Stomatology, Department of General Dentistry, State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Chengdu, China.

出版信息

J Appl Oral Sci. 2025 May 30;33:e20240524. doi: 10.1590/1678-7757-2024-0524. eCollection 2025.

DOI:10.1590/1678-7757-2024-0524

PMID:40465950

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12218195/

Abstract

OBJECTIVES

To investigate the effects of sodium fluoride (NaF), casein phosphopeptide-amorphous calcium phosphate (CPP-ACP), and titanium tetrafluoride (TiF4) on the prevention of demineralization in irradiated bovine enamel and dentin in vitro.

METHODOLOGY

The enamel and dentin sample blocks were subjected to 50 Gy of radiation and divided into five groups (n=13): the deionized distilled water group, the NaF group, the CPP-ACP group, the NaF + CPP-ACP group, and the TiF4 group. After being treated with various materials for 30 minutes, the samples were remineralized for 12 hours and demineralized for 48 hours. The samples were then evaluated by scanning electron microscope (SEM), atomic force microscope (AFM), energy dispersive spectrometer (EDS), and transverse microradiography (TMR). Data were analyzed by ANOVA and the Kruskal-Wallis H test (α=0.05).

RESULTS

SEM and TMR indicated that the TiF4 group promoted more mineral deposits on the enamel and dentin samples, showing the least mineral loss and the lowest lesion depth. AFM results showed that the NaF + CPP-ACP group had the lowest enamel roughness (p<0.05), whereas the TiF4 group showed the lowest roughness in dentin samples (p<0.05). EDS showed that titanium (Ti) was deposited on the surface of the TiF4 group samples, whereas the NaF + CPP-ACP group more greatly aggregated fluorine.

CONCLUSION

TiF4 significantly impacted the prevention of demineralization in irradiated dental hard tissues. Combining NaF and CPP-ACP more effectively prevented demineralization than either agent used alone.

摘要

目的

研究氟化钠（NaF）、酪蛋白磷酸肽 - 无定形磷酸钙（CPP - ACP）和四氟化钛（TiF4）对体外照射牛牙釉质和牙本质脱矿预防作用的影响。

方法

将牙釉质和牙本质样本块进行50 Gy的辐射，并分为五组（n = 13）：去离子蒸馏水组、NaF组、CPP - ACP组、NaF + CPP - ACP组和TiF4组。用各种材料处理30分钟后，样本再矿化12小时，然后脱矿48小时。随后通过扫描电子显微镜（SEM）、原子力显微镜（AFM）、能量色散谱仪（EDS）和横向显微放射照相术（TMR）对样本进行评估。数据采用方差分析和Kruskal - Wallis H检验进行分析（α = 0.05）。

结果

SEM和TMR表明，TiF4组在牙釉质和牙本质样本上促进了更多的矿物质沉积，显示出最少的矿物质损失和最低的病变深度。AFM结果表明，NaF + CPP - ACP组牙釉质粗糙度最低（p < 0.05），而TiF4组在牙本质样本中显示出最低的粗糙度（p < 0.05）。EDS显示，Ti沉积在TiF4组样本表面，而NaF + CPP - ACP组氟的聚集程度更高。