CPP-ACP 联合氟化钠对牛牙釉质脱矿抑制作用的定量评估：micro-CT 研究

Effects of CPP-ACP with sodium fluoride on inhibition of bovine enamel demineralization: a quantitative assessment using micro-computed tomography.

机构信息

Cariology and Operative Dentistry, Department of Restorative Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8549, Japan.

出版信息

J Dent. 2011 Jun;39(6):405-13. doi: 10.1016/j.jdent.2011.03.005. Epub 2011 Mar 29.

DOI:10.1016/j.jdent.2011.03.005

PMID:21453746

Abstract

OBJECTIVES

The present study evaluated the effects of casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) and CPP-ACP with 900 ppm fluoride (CPP-ACPF) pastes on inhibition of enamel demineralization over time, using polychromatic micro-computed tomography (micro-CT).

METHODS

Enamel blocks were prepared from bovine teeth. The specimens were each treated by one of the following agents, 30 min daily for 7 days: deionized water (negative control); CPP-ACP paste; CPP-ACPF paste; and NaF solutions (positive controls) (90, 900, and 9000 ppm F). After treatment, the specimens were immersed in a demineralizing solution (pH 4.5) for 24, 72, and 120 h. Mean mineral loss (ML) and lesion depth (LD) after each period were determined from mineral density profiles obtained using micro-CT.

RESULTS

ML values in all the treatment groups were significantly smaller than those in the control group after 72 and 120 h of demineralization (p < 0.05, two-way ANOVA and t-test with Bonferroni correction). ML values in CPP-ACPF and NaF solution groups were significantly smaller compared to CPP-ACP group after 72 h (p < 0.05). LD values in the CPP-ACPF and all the NaF solutions groups were significantly smaller compared to the control group after 120 h (p < 0.05). The 9000 ppm F group showed the lowest nominal ML and LD values.

CONCLUSIONS

The application of CPP-ACP or CPP-ACPF pastes to sound enamel surfaces resulted in inhibition of enamel demineralization, and a better effect was noted for the latter paste. Quantitative assessment using polychromatic micro-CT demonstrated to be useful for detecting mineral density changes.

摘要

目的

本研究采用多色微计算机断层扫描（micro-CT）评价酪蛋白磷酸肽-无定形磷酸钙（CPP-ACP）和含 900 ppm 氟化物的 CPP-ACP（CPP-ACPF）糊剂对抑制 enamel脱矿的长期效果。

方法

从牛牙制备牙釉质块。每天用以下一种试剂处理标本 30 分钟，共 7 天：去离子水（阴性对照）；CPP-ACP 糊剂；CPP-ACPF 糊剂；以及 NaF 溶液（阳性对照）（90、900 和 9000 ppm F）。处理后，将标本浸入脱矿液（pH 4.5）中 24、72 和 120 h。使用 micro-CT 获得的矿物密度谱确定每个时期的平均矿物损失（ML）和病变深度（LD）。

结果

在脱矿 72 和 120 h 后，所有治疗组的 ML 值均显著小于对照组（p < 0.05，双因素方差分析和 Bonferroni 校正后的 t 检验）。与 CPP-ACP 组相比，CPP-ACPF 组和所有 NaF 溶液组在 72 h 后的 ML 值显著更小（p < 0.05）。在 120 h 后，CPP-ACPF 组和所有 NaF 溶液组的 LD 值均显著小于对照组（p < 0.05）。9000 ppm F 组的名义 ML 和 LD 值最低。