Dündar Ayşe, Yılmaz Kaan, Barutçugil Çağatay, Koyuncu Özyurt Özlem
Department of Restorative Dentistry, Faculty of Dentistry, Akdeniz University, Antalya, 07058, Turkey.
Edirne Ağız ve Diş Sağlığı Merkezi, Edirne, Turkey.
Clin Oral Investig. 2025 Jun 25;29(7):353. doi: 10.1007/s00784-025-06443-w.
This study aimed to evaluate the effects of laser application with different agents on dentin surface roughness, tubule occlusion, and bacterial adhesion in vitro.
A total of 216 dentin blocks from bovine incisors were divided into nine groups. Treatments included 5% sodium fluoride (NaF) varnish, casein phosphopeptide-amorphous calcium phosphate (CPP-ACP), Er, Cr: YSGG laser, and diode laser in various combinations. Surface roughness (SR) was measured using a profilometer. S. mutans and S. mitis suspensions were incubated for 24 h, and bacterial adhesion was quantified (×10⁸ Cfu/ml) and visualized using confocal laser scanning microscopy. Tubule occlusion was assessed via SEM imaging and analyzed with ImageJ software. Statistical analysis was performed using one-way ANOVA and Tukey HSD (p = 0.05).
Except for the CPP-ACP groups treated with laser, all groups exhibited significantly higher surface roughness (p < 0.05); however, this did not influence bacterial adhesion (p > 0.05). All treatments significantly reduced the number and diameter of open dentinal tubules (p < 0.05). The combination of NaF with Er, Cr: YSGG and diode laser enhanced tubule occlusion, while CPP-ACP with laser had no additional effect.
Increased surface roughness, except in CPP-ACP with laser groups, did not affect bacterial adhesion. All desensitization methods effectively promoted tubule occlusion, with the diode laser demonstrating greater efficacy than the Er, Cr: YSGG laser.
Lasers should be used with caution as they may increase the roughness of the dentin surface. The combined application of NaF with lasers may improve its clinical efficacy.
本研究旨在评估不同药物联合激光应用对牙本质表面粗糙度、小管封闭及体外细菌黏附的影响。
将216个牛切牙牙本质块分为9组。处理方式包括5%氟化钠（NaF）清漆、酪蛋白磷酸肽 - 无定形磷酸钙（CPP - ACP）、铒铬：钇 - 钪 - 镓石榴石（Er, Cr: YSGG）激光和二极管激光的各种组合。使用轮廓仪测量表面粗糙度（SR）。将变形链球菌和缓症链球菌悬液孵育24小时，对细菌黏附进行定量（×10⁸ 菌落形成单位/毫升），并使用共聚焦激光扫描显微镜进行观察。通过扫描电子显微镜（SEM）成像评估小管封闭情况，并使用ImageJ软件进行分析。采用单因素方差分析和Tukey HSD检验进行统计学分析（p = 0.05）。
除激光处理的CPP - ACP组外，所有组的表面粗糙度均显著更高（p < 0.05）；然而，这并未影响细菌黏附（p > 0.05）。所有处理均显著减少了开放牙本质小管的数量和直径（p < 0.05）。NaF与Er, Cr: YSGG激光和二极管激光联合使用增强了小管封闭，而CPP - ACP与激光联合使用则无额外效果。
除激光处理且含CPP - ACP的组外，表面粗糙度增加并不影响细菌黏附。所有脱敏方法均有效促进了小管封闭，二极管激光的效果优于Er, Cr: YSGG激光。
激光使用时应谨慎，因为它们可能会增加牙本质表面的粗糙度。NaF与激光联合应用可能会提高其临床疗效。
