复杂多参数流式细胞术技术的进展：在干细胞研究中的应用。
Advances in complex multiparameter flow cytometry technology: Applications in stem cell research.
作者信息
Preffer Frederic, Dombkowski David
机构信息
Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA.
出版信息
Cytometry B Clin Cytom. 2009 Sep;76(5):295-314. doi: 10.1002/cyto.b.20480.
Abstract
Flow cytometry and cell sorting are critical tools in stem cell research. Recent advances in flow cytometric hardware, reagents, and software have synergized to permit the stem cell biologist to more fully identify and isolate rare cells based on their immunofluorescent and light scatter characteristics. Some of these improvements include physically smaller air-cooled lasers, new designs in optics, new fluorescent conjugate-excitation pairs, and improved software to visualize data, all which combine to open up new horizons in the study of stem cells, by enhancing the resolution and specificity of inquiry. In this review, these recent improvements in technology will be outlined and important cell surface and functional antigenic markers useful for the study of stem cells described.
摘要
流式细胞术和细胞分选是干细胞研究中的关键工具。流式细胞仪硬件、试剂和软件的最新进展协同作用，使干细胞生物学家能够根据细胞的免疫荧光和光散射特性更全面地识别和分离稀有细胞。其中一些改进包括体积更小的风冷激光器、光学方面的新设计、新的荧光共轭激发对以及用于可视化数据的改进软件，所有这些结合起来，通过提高研究的分辨率和特异性，为干细胞研究开辟了新的视野。在这篇综述中，将概述这些技术上的最新改进，并描述对干细胞研究有用的重要细胞表面和功能性抗原标志物。
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