De Ávila Juliette, Ramos-Casallas Alejandro, Velandia-Romero Myriam Lucia, Perdomo-Lara Sandra Janneth, Acero-Molina Diana, Florez-Sarmiento Cristian, Parra-Izquierdo Viviana, Castellanos Jaime E, Chila-Moreno Lorena, Bautista-Molano Wilson, Beltran-Ostos Adriana, Jaimes Diego Alejandro, Bello-Gualtero Juan Manuel, Pacheco-Tena Cesar, Chalem Philippe, Romero-Sánchez Consuelo
Cellular and Molecular Immunology Group - InmuBo-, School of Dentistry, Universidad El Bosque, Av. Cra 9 No. 131 A-02, Bogotá, Colombia.
Grupo de Virología, Universidad El Bosque, Bogotá, Colombia.
Sci Rep. 2025 Jun 4;15(1):19517. doi: 10.1038/s41598-025-03532-4.
Spondyloarthritis (SpA) correlates with elevated serum secretory-IgA (sSIgA). Retrotranscytosis, mediated by CD71 and Dectin-1, is linked to high sSIgA levels. This research investigated retrotranscytosis in the gut of SpA patients and its relationship with sSIgA, ileocolonoscopic findings, and activity indices. A cohort of 82 was derived from 180 patients based on selection criteria; 41 consented to DCE colonoscopy assessment. Measurements included CD71, Dec-1, sSIgA, and disease scores. Samples exhibiting apical CD71, Dec-1, or both were analysed for receptor/SIgA interactions. Apical CD71 and Dec-1 were predominantly found in the ileum. Multivariate analyses indicated CD71's association with heightened sSIgA (p = 0.05) and ASDAS-CRP (p = 0.036; OR 1.71; 95% CI 1.00-3.11), Dec-1's correlation with ileal inflammation (p = 0.05; OR 3.06; 95% CI 1.00-10.01), and the CD71/Dec-1 relationship with ileal villi atrophy (p = 0.038; OR 4.24; 95% CI 1.00-18.88). Microscopic evaluations demonstrated interactions between CD71/SIgA but not between CD71/Dec-1 or Dec/SIgA. The localised expression of CD71 and its co-localisation with Dec-1 may contribute to systemic increases in serum SIgA and activity scores in SpA patients. The findings suggest that sSIgA levels may serve as a non-invasive biomarker for gastrointestinal involvement in SpA and that retrotranscytosis could be a key mechanism in the gut-joint axis of SpA.
脊柱关节炎（SpA）与血清分泌型IgA（sSIgA）升高相关。由CD71和Dectin-1介导的逆向转运与高sSIgA水平有关。本研究调查了SpA患者肠道中的逆向转运及其与sSIgA、回结肠镜检查结果和活动指数的关系。根据选择标准，从180名患者中选取了82名组成队列；41名患者同意进行双对比结肠镜评估。测量指标包括CD71、Dec-1、sSIgA和疾病评分。对显示顶端CD71、Dec-1或两者均有的样本进行受体/SIgA相互作用分析。顶端CD71和Dec-1主要在回肠中发现。多变量分析表明，CD71与sSIgA升高相关（p = 0.05）以及与ASDAS-CRP相关（p = 0.036；OR 1.71；95% CI 1.00 - 3.11），Dec-1与回肠炎症相关（p = 0.05；OR 3.06；95% CI 1.00 - 10.01），以及CD71/Dec-1与回肠绒毛萎缩相关（p = 0.038；OR 4.24；95% CI 1.00 - 18.88）。显微镜评估显示CD71/SIgA之间存在相互作用，但CD71/Dec-1或Dec/SIgA之间不存在相互作用。CD71的局部表达及其与Dec-1的共定位可能导致SpA患者血清SIgA和活动评分的全身性升高。研究结果表明，sSIgA水平可能作为SpA患者胃肠道受累的非侵入性生物标志物，并且逆向转运可能是SpA肠道-关节轴中的关键机制。
