Pierzynowski Stefan G, Wychowański Piotr, Szczesny Wiesław, Galloto Robert, Zaworski Kamil, Szkopek Dominika, Woliński Jarosław, Donaldson Janine, Pierzynowska Kateryna
Department of Biology, Lund University, Lund, Sweden.
Anara AB, Trelleborg, Sweden.
Sci Rep. 2025 Jun 4;15(1):19704. doi: 10.1038/s41598-025-04625-w.
The recognition of amine groups by ninhydrin, along with a simple mathematical algorithm, showed that di- and tripeptides derived from dietary protein are the major end products of protein digestion entering the blood postprandially. There are thousands of oligopeptides appearing in the gut during protein digestion. However, the presented study on a pig model clearly shows that peptides longer than tri-amino acid peptides do not appear postprandially in the blood in nutritional amounts. We hypothesize that the measurement of postprandial free amino acids, di- and tripeptides and proportions between these components could be a useful, desirable tool both in laboratory and clinical practice, which could be used to determine the metabolic importance of protein digestion end products in health and disease.
茚三酮对胺基的识别，结合一种简单的数学算法，表明源自膳食蛋白质的二肽和三肽是餐后进入血液的蛋白质消化的主要终产物。蛋白质消化过程中肠道内会出现数千种寡肽。然而，目前对猪模型的研究清楚地表明，长度超过三氨基酸肽的肽在餐后不会以营养量出现在血液中。我们推测，测定餐后游离氨基酸、二肽和三肽以及这些成分之间的比例，在实验室和临床实践中可能都是一种有用且理想的工具，可用于确定蛋白质消化终产物在健康和疾病中的代谢重要性。
