Department of Life Sciences, National Central University, No. 300 Chung-da Rd., Chung-li, 32001, Taiwan, ROC.
Bioresour Technol. 2010 Apr;101(8):2853-9. doi: 10.1016/j.biortech.2009.11.029. Epub 2009 Dec 30.
Pseudomonas sp. TX1, is able to use octylphenol polyethoxylates (OPEO(n), or Triton X-100; average n = 9.5) as a sole carbon source. It can grow on 0.05-20% of OPEO(n) with a specific growth rate of 0.34-0.44 h(-1). High-performance liquid chromatography-mass spectrometer analysis of OPEO(n) degraded metabolites revealed that strain TX1 was able to shorten the ethoxylate chain and produce octylphenol (OP). Furthermore, formation of the short carboxylate metabolites, such as carboxyoctylphenol polyethoxylates (COPEO(n), n = 2, 3) and carboxyoctylphenol polyethoxycarboxylates (COPEC(n), n = 2, 3) began at the log stage, while octylphenol polyethoxycarboxylates (OPEC(n), n = 1-3) was formed at the stationary phase. All the short-ethoxylated metabolites, OPEO(n), OPEC(n), COPEO(n), and COPEC(n), accumulated when the cells were in the stationary phase. This study is the first to demonstrate the formation of COPEO(n) and COPEC(n) from OPEO(n) by an aerobic bacterium.
铜绿假单胞菌 TX1 能够利用辛基酚聚氧乙烯醚（OPEO(n)，或曲通 X-100；平均 n = 9.5）作为唯一碳源。它可以在 0.05-20%的 OPEO(n)中生长，比生长速率为 0.34-0.44 h(-1)。对 OPEO(n)降解代谢产物的高效液相色谱-质谱分析表明，TX1 菌株能够缩短乙氧基链并产生辛基酚（OP）。此外，短羧酸代谢物的形成，如羧基辛基酚聚氧乙烯醚（COPEO(n)，n = 2,3）和羧基辛基酚聚氧羧酸酯（COPEC(n)，n = 2,3），在对数阶段开始形成，而辛基酚聚氧羧酸酯（OPEC(n)，n = 1-3）则在稳定阶段形成。所有短乙氧基化代谢物，OPEO(n)、OPEC(n)、COPEO(n)和 COPEC(n)，在细胞处于稳定期时积累。本研究首次证明了好氧菌从 OPEO(n) 形成 COPEO(n)和 COPEC(n)。
