Du Jianfeng, Li Heng, Liu Shenjie, Wu Limin, Liu Yiming, Li Yue, Zhao Xin, Gao Qixiong, Dong Jing, Lei Chen, Sun Fuxin, Tian Xueliang, Wang Chuanjun, Lu Ninghai, Jiao Yang
School of Resources and Environment, Henan Institute of Science and Technology, Xinxiang, P. R. China.
College of Chemistry and Material Science, Shandong Agricultural University, Tai'an, P. R. China.
Pest Manag Sci. 2025 Sep;81(9):5134-5145. doi: 10.1002/ps.8868. Epub 2025 Jun 4.
The application of biocontrol agents offers a promising strategy for managing pear anthracnose, yet their efficacy is often limited by unstable colonization on fruit surfaces.
Here, we isolated Paenibacillus polymyxa JF_P68, which exhibits strong inhibitory activity against Colletotrichum fructicola. To enhance its environmental tolerance, we developed a hyaluronic acid (HA)-functionalized nanoarmor using a layer-by-layer coating technique. This nanoarmor significantly improved the JF_P68's resistance to ultraviolet radiation and oxidative stress. In vivo trials demonstrated that nanoarmor-equipped JF_P68 reduced pear anthracnose lesion diameters by 43.5% and maintained higher colonization levels on fruit surfaces. Genome analysis revealed 20 biosynthetic gene clusters, including novel antimicrobial candidates, underpinning its broad-spectrum antifungal activity.
The single-cell coating method, characterized by simplicity, scalability, and biocompatibility, significantly enhances the persistence and efficacy of biocontrol agents. This study provides a novel approach to improving the biological control of pear anthracnose. © 2025 Society of Chemical Industry.
生物防治剂的应用为梨炭疽病的防治提供了一种有前景的策略，但其效果往往受到在果实表面定殖不稳定的限制。
在此，我们分离出了多粘芽孢杆菌JF_P68，它对胶孢炭疽菌表现出强烈的抑制活性。为提高其环境耐受性，我们采用层层包覆技术开发了一种透明质酸（HA）功能化的纳米铠甲。这种纳米铠甲显著提高了JF_P68对紫外线辐射和氧化应激的抗性。体内试验表明，配备纳米铠甲的JF_P68使梨炭疽病病斑直径减小了43.5%，并在果实表面保持了更高的定殖水平。基因组分析揭示了20个生物合成基因簇，包括新型抗菌候选基因，这是其广谱抗真菌活性的基础。
单细胞包覆方法具有简单、可扩展和生物相容性的特点，显著提高了生物防治剂的持久性和效果。本研究为改善梨炭疽病的生物防治提供了一种新方法。© 2025化学工业协会。
